高等学校化学学报 ›› 2017, Vol. 38 ›› Issue (5): 738-742.doi: 10.7503/cjcu20170105

• 分析化学 • 上一篇    下一篇

牛油果中化学成分的内部萃取电喷雾电离质谱检测

柯牡芳2, 韩京2, 朱腾高3, 刘文杰1, 张华2, 寇伟2, 梁大鹏1()   

  1. 1. 吉林大学环境与资源学院, 地下水资源与环境教育部重点实验室, 长春130012
    2. 吉林大学化学学院, 无机合成与制备化学国家重点实验室, 长春130012
    3. 东华理工大学江西省质谱科学与仪器重点实验室, 南昌330013
  • 收稿日期:2017-02-13 出版日期:2017-05-10 发布日期:2017-04-20
  • 作者简介:联系人简介: 梁大鹏, 男, 博士, 副教授, 主要从事环境化学方面的研究. E-mail:liangdp@jlu.edu.cn
  • 基金资助:
    国家自然科学基金(批准号: 21307040, 21427802)和江西省质谱科学与仪器重点实验室开放基金(批准号: JXMS201601)资助

Direct and Rapid Analysis of Avocado Using Internal Extractive Electrospray Ionization Mass Spectrometry

KE Mufang2, HAN Jing2, ZHU Tenggao3, LIU Wenjie1, ZHANG Hua2, KOU Wei2, LIANG Dapeng1,*()   

  1. 1. College of Environment and Resources, Key Lab of Groundwater Resources and Environment, Ministry of Education, Jilin University, Changchun 130012, China
    2. State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun 130012, China
    3. Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation,East China Institute of Technology, Nanchang 330013, China
  • Received:2017-02-13 Online:2017-05-10 Published:2017-04-20
  • Contact: LIANG Dapeng E-mail:liangdp@jlu.edu.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(Nos.21307040, 21427802) and the Open Found of Jiangxi Provincial Key Laboratory for Mass Spectrometry and Instrumentation, China(No.JXMS201601)

摘要:

采用内部萃取电喷雾电离质谱(iEESI-MS)技术, 以甲醇作为萃取溶剂, 无需样品预处理, 在优化条件下分别对牛油果果肉和果皮中化学成分进行快速直接鉴定, 并考察了不同成熟度牛油果果肉中化学成分的差异. 实验结果表明, 在负离子检测模式下, 从果肉中鉴定出棕榈酸、 硬脂酸、 棕榈油酸、 油酸和亚麻酸等12种有效营养成分, 从果皮中鉴定出儿茶素、 绿原酸等11种营养成分. 利用正交偏最小二乘判别分析法(OPLS-DA)对不同成熟度的牛油果果肉的iEESI-MS指纹谱图数据进行分析发现, 此方法能够有效判别不同成熟程度的牛油果且5种化学成分差异显著. 本方法无需样品预处理、 样品耗量少、 分析速度快(单个样品检测时间小于1 min)且操作简便, 为植物有效营养成分和医用价值的开发提供了一种快速质谱分析新方法.

关键词: 牛油果, 内部萃取电喷雾电离质谱, 化学成分, 成熟程度

Abstract:

Avocado plays significant roles in food and medicine industries, due to its advantages of high nutrition profile, low sugar and high energy. In this work, the chemical compositions of pulp and peel of avocado were fast analyzed by internal extraction electrospray ionization mass spectrometry technology(iEESI-MS) without any pretreatment. Furthermore, Orthogonal partial least squares discriminant analysis(OPLS-DA) model was established to distinguish avocado’s maturity degree between unripening and ripening. The experimental results indicated that in negative ion mode, a total of 12 compounds such as palmitic acid, linolenic acid, palmitic acid, linolenic acid, oleic acid, stearic acid in pulp of avocado were indentified; while 11 compounds in peel of avocado were indentified including cianidanol, chlorogenic acid, pantothenic acid and so on. The differences of chemical compounds between unripening and ripening could be obviously observed through the model of OPLS-DA. The merits of this method to analyze compounds of avocado by iEESI-MS include simplicity, excellent specificity, high throughput and minimum sample pretreatment.

Key words: Avocado, Internal extraction electrospray ionization mass spectrometry, Composition, Maturity

中图分类号: