高等学校化学学报

高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (12): 2488.

• 研究论文 • 上一篇    下一篇

分子印迹微凝胶模拟酶的研究

王红飞1,2,唐春燕1,杨浩1,张黎明1,2   

  1. 1. 中山大学化学与化学工程学院, 新型聚合物材料设计合成与应用广东省高校重点实验室, 广州 510275
    2. 中山大学聚合物复合材料及功能材料教育部重点实验室, 广州 510275
  • 收稿日期:2010-02-23 修回日期:2010-05-17 出版日期:2010-12-10 发布日期:2010-12-06
  • 通讯作者: 张黎明 E-mail:ceszhlm@mail.sysu.edu.cn
  • 作者简介:张黎明, 男, 博士, 教授, 博士生导师, 主要从事生物功能材料的研究.
  • 基金资助:

    国家自然科学基金(批准号: 20874116, 20676155)和教育部高等学校博士点基金(批准号: 20090171110023)资助.

Study on Enzyme Mimics of Molecularly Imprinted Microgels

WANG Hong-Fei1,2, TANG Chun-Yan1, YANG Hao1, ZHANG Li-Ming1,2*   

  1. 1. Key Laboratory of Designed Synthesis and Application of Polymer Materials, Sun Yat\|Sen University, Guangzhou 510275, China
    2. Key Laboratory for Polymeric Composite and Functional Materials, Ministry of Education, School of Chemistry and Chemical Engineering, Sun Yat\|Sen University, Guangzhou 510275, China
  • Received:2010-02-23 Revised:2010-05-17 Online:2010-12-10 Published:2010-12-06
  • Contact: Li-Ming Zhang E-mail:ceszhlm@mail.sysu.edu.cn
  • About author:张黎明, 男, 博士, 教授, 博士生导师, 主要从事生物功能材料的研究.
  • Supported by:

    国家自然科学基金(批准号: 20874116, 20676155)和教育部高等学校博士点基金(批准号: 20090171110023)资助.

PDF (PC)

被引次数

32

摘要： 采用油包水反相乳液法, 以马来酸酐酯化葡聚糖-氨基吡啶偶联物(Dex-MA-AP)为功能大单体、过渡态类似物p-硝基苯磷酸酯(NPP)为模板分子、Co2+为中心离子, 制得分子印迹微凝胶模拟酶(MIGs). 用紫外光谱研究了Dex-MA-AP上吡啶功能基团与NPP之间的相互作用. 用SEM观察了MIGs的形貌和大小. 研究发现, MIGs催化活性受模板分子和交联剂用量的影响，MIGs催化p-硝基乙酸苯酯(NPA)水解反应行为可用Michaelis-Menten方程进行描述, 其最大催化水解反应速率(Vm)和Michaelis-Menten常数(Km)分别为25.1 nmol/h和0.030 mmol/L, 且具有较好的催化选择性.

关键词: 模拟酶, 分子印迹, 微凝胶, 催化活性, 催化选择性

Abstract: Molecularly imprinted microgels (MIGs) exhibiting selective esterase activity were prepared by a reverse emulsion method using maleic anhydride esterified dextran-aminopyridine conjugate (Dex-MA-AP) as the functional macromonomer, p-nitrophenyl phosphate (NPP) as the stable transition state analogue (TSA) and Co2+ as the coordination center. The interaction between Dex-MA-AP and NPP was investigated by UV adsorption spectra. The morphology and size of MIGs were observed by scanning electron micrographs (SEM). It was found that the catalytic activity of MIGs was greatly influenced by the amounts of the template and cross-linking agent. The hydrolysis kinetics of p-nitrophenyl acetate (NPA) in the presence of MIGs could be described by the Michaelis-Menten equation. The maximium velocity (Vm) and Michaelis-Menten constant (Km) were found to be 25.1 mmol/h and 0.030 mmol/L, respectively. In addition, the MIGs was found to show high catalytic selectivity to NPA.

Key words: Enzyme mimics, Molecularly imprinting, Microgels, Catalytic activity, Catalytic selectivity

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