基于PbS QDs/TiO2 NPs构建新型谷胱甘肽光电化学传感器

doi:10.7503/cjcu20190106

摘要/Abstract

摘要：

通过高温煅烧将二氧化钛纳米颗粒(TiO₂ NPs)修饰到ITO电极表面制成TiO₂ NPs/ITO电极, 再采用连续离子层吸附反应(SILAR)循环将硫化铅量子点(PbS QDs)修饰到TiO₂/ITO电极表面制得PbS QDs/TiO₂ NPs/ITO电极, 并将该电极应用于检测谷胱甘肽(GSH)的光电化学传感器. 在该传感器中, 当PbS QDs受470 nm可见光的激发时将产生电子(e)和光生空穴(h ⁺), 光生空穴可被溶液中的GSH捕获, 并将GSH氧化成GSSH, 有效避免电子和空穴的复合, 显著提高了光电效率. 该传感器对GSH的检测具有较高的灵敏度和选择性, 线性检测范围为0.06~1 mmol/L, 检出限(LOD)为4.6×10 ^-3 mmol/L(S/N=3).

关键词: 硫化铅量子点, 二氧化钛纳米颗粒, 谷胱甘肽, 光电化学, 生物传感器

Abstract:

Firstly, titanium dioxide nanoparticles(TiO₂ NPs) was modified to the surface of indium tin oxide(ITO) electrode by high-temperature calcination to prepare TiO₂ NPs/ITO electrode. And then sulfide quantum dots(PbS QDs) were modified to the surface of TiO₂ NPs/ITO electrode by successive ionic layer adsorption and reaction(SILAR) cycle to prepare the PbS QDs/TiO₂ NPs/ITO electrode. And it was used to detect glutathione(GSH). In this sensor, when PbS QDs are excited by 470 nm visible light, it will produce electrons(e) and holes(h ⁺). Immediately after, h ⁺ will be captured by GSH in solution, and then GSH is oxidized into GSSH. Therefore, the recombination of electrons and holes were avoided effectively. Thus the photoelectric efficiency has been significantly improved. This sensor had satisfactory sensitivity and selectivity for GSH. what’s more, the detection range is 0.06—1 mmol/L, and the detection limit(LOD) is 4.6×10 ^-3 mmol/L(S/N=3).

Key words: PbS QDs, TiO₂ NPs, Glutathione(GSH), Photoelectrochemical, Biosensor

中图分类号:

O652.1

TrendMD:

郑姗, 刘洋, 陈飘飘, 邢怡晨, 黄朝表. 基于PbS QDs/TiO₂ NPs构建新型谷胱甘肽光电化学传感器. 高等学校化学学报, 2019, 40(9): 1866.

ZHENG Shan, LIU Yang, CHEN Piaopiao, XING Yichen, HUANG Chaobiao. Novel Glutathione Photoelectrochemical Sensor Based on PbS QDs/TiO₂ NPs ^†. Chem. J. Chinese Universities, 2019, 40(9): 1866.

图/表 13

Fig.1 SEM images of TiO2 NPs/ITO(A) and PbS QDs/TiO2 NPs/ITO(B) electrodes

Fig.2 XRD patterns of ITO(a), TiO2 NPs/ITO(b) and PbS QDs/TiO2 NPs/ITO(c) electrodes

Fig.3 Full-scan XPS spectra of TiO2 NPs and PbS QDs/TiO2 NPs electrodes(A) and high-resolution XPS spectra of Ti2p(B), O1s(C), Pb4f(D) and S2p(E) of PbS QDs/TiO2 NPs electrode

Fig.4 EIS of TiO2 NPs/ITO(a) and PbS QDs/TiO2 NPs/ITO(b) electrodes The EIS measurements are carried out in 0.1 mol/L KCl containing 5.0 mmol/L K3Fe(CN)6/K4Fe(CN)6(1∶1), The frequency range was between 0.1 and 1×105 Hz with an applied voltage of 5 mV. Inset: equivalent circuit diagram. Cd: capacitance; Rct: charge transfer resistance; Ru: ohmic resistance; Zw: diffusion resistance.

Fig.5 Effect of TiO2 NPs concentration on photocurrent responses of the sensor

Fig.6 Effect of SILAR cycle times on photocurrent responses of the sensor

Fig.7 Effect of wavelength on photocurrent responses of the sensor

Fig.8 Effect of potential on photocurrent responses of the sensor

Fig.9 Photocurrent response curves of different modified ITO(a), TiO2/ITO(b), PbS QDs/TiO2/ITO(c) and QDs/TiO2/ITO(d) electrodes in 0.2 mmol/L GSH/PbS

Fig.10 Preparation process diagram(A) and mechanism diagram(B) of GSH sensor

Fig.11 Linear calibration curve GSH sensor Inset: Photocurrent response of GSH sensor.

Fig.12 Stability of PbS QDs/TiO2 NPs/ITO electrode

Fig.13 Photocurrent signals of the GSH sensor toward in the presence of 1 mmol/L cysteine(Cys), alanine(Ala), dopamine(DA), glycine(Gly), leucine(Lue), arginine(Arg), valine(Val), glucose(Glu) and all their mixture(Mix) and 0.2 mmol/L GSH

参考文献 39

[1]	Zhang X., Liu M., Liu H., Zhang S., ,. Biosens. Bioelectron. 2014, 56, 307— 312
[2]	Zhao W. W., Xu J. J., Chen H. Y., ,. Chem. Rev. 2014, 114, 7421— 7441
[3]	Zhang X., Guo Y., Liu M., Zhang S., ,. RSC Adv. 2013, 3, 2846— 2857
[4]	Wu Y., Zhang B., Guo L. H., ,Anal. Chem., 2013, 85, 6908— 6914
[5]	Li H., Xiao Q., Lv J. X., Lei Q., Huang Y. J., ,Anal. Biochem., 2017, 531, 48— 55
[6]	Emamdoust A. S., Farjami S., ,. J. Alloy. Compd. 2018, 738, 432— 439
[7]	Babamiri B., Hallaj R., Salimi A., ,Biosens. Bioelectron., 2018, 99, 353— 360
[8]	Peng C., Wang W., Zhang W., Liang Y., Zhuo L., ,Appl. Surf. Sci., 2017, 420, 286— 295
[9]	Subramanian A., Pan Z., Li H., Zhou L., Li W., Qiu Y., Xu Y., Hou Y., Zhang Y., ,. Appl. Surf. Sci. 2017, 420, 631— 637
[10]	Dong W., Li H., Xi J., Mu J., Huang Y., Ji Z., Wu X., ,. J. Alloy. Compd. 2017, 724, 280— 286
[11]	Ye M., Xin X., Lin C., Lin Z., ,Nano Lett., 2011, 11, 3214— 3220
[12]	Lv M., Zheng D., Ye M., Sun L., Xiao J., Guo W Lin C ., Nanoscale, 2012, 4, 5872— 5879
[13]	Wang Y. J., Han C., Liu B., Yang P. D., ,. ACS Nano 2012, 6, 5060— 5069
[14]	Stankovich S., Dikin D. A., Piner R. D., Kohlhaas K. A., Kleinhammes A., Jia Y., Wu Y., Nguyen S. T., Ruoff R. S ., Carbon, 2007, 45, 1558— 1565
[15]	Mor G K .,Varghese O. K., Paulose M., Shankar K., Grimes C. A., Sol. Energ. Mat. Sol. C, 2006, 90, 2011— 2075
[16]	Podporska-Carroll J., Panaitescu E., Quilty B., Wang L., Menon L., Pillai S. C., ,. Appl. Catal. B: Environ. 2015, 176, 70— 75
[17]	Qiu B., Xing M., Zhang J., ,J. Am. Chem. Soc., 2014, 136, 5852— 5855
[18]	Jin L. Y., Dong Y. M., Wu X. M., Cao G. X., Wang G. L., ,Anal. Chem., 2015, 87, 10429— 10436
[19]	Yu Y., Zhang K., Sun S., ,Appl. Surf. Sci., 2012, 258, 7181— 7187
[20]	Li F., Huang X. T., Kong T., Liu X. Q., Qin Q. H., Li Z., ,J. Alloy. Compd., 2009, 485, 554— 560
[21]	Li Y., Wu P., Xu H., Zhang H., Zhong X ., Analyst, 2011, 136, 196— 200
[22]	Franco R., Panayiotidis M. I., Cidlowski J. A., ,J. Biol. Chem., 2007, 282, 30452— 30465
[23]	Wang L. M., Wei M., Gu X. Q., Zhao Y. L., Qiang Y. G., ,J. Electron. Mater., 2018, 47( 11), 6540— 6550
[24]	Zhang H., Xia X., Zhao H., Zhang G. H., Jiang D. Y., Xue X. Y., Zhang J., ,. Dyes Pigments 2019, 163, 183— 189
[25]	Rani P. J., Vishnuvardhan C., Nimbalkar R. D., Garg P., Satheeshkumar N., ,. J. Pharm. Biomed. Anal. 2018, 155, 320— 328
[26]	Shi M., Huang Y., Zhao J. J., Li S. T., Liu Y. J., Zhao S. L ., Talanta, 2018, 179, 466— 471
[27]	Wang L. M., Gu X. Q., Zhao Y. L., Wei M., Qiang Y. H., Zhao Y., ,. J. Mater. Sci-Mater. El. 2018, 29, 19278— 19286
[28]	Lee H., Leventis H. C., Moon S. J., Chen P., Ito S., Haque S. A., Torres T., Nüesch F., Geiger T., Zakeeruddin S. M., Grätzel M., Nazeeruddin M. K., ,. Adv. Funct. Mater. 2009, 19, 2735— 2742
[29]	Wang D. F., Zhao H. G., Wu N. Q., Khakani M. A. E., Ma D. L., ,. J. Phys. Chem. Lett. 2010, 1, 1030— 1035
[30]	Mali S. S., Desai S. K., Kalagi S. S., Betty C. A., Bhosale P. N., Devan R. S., Ma Y. R., Patil P. S., ,. Dalton Trans. 2012, 41, 6130— 6136
[31]	Nagaveni K., Hegde M. S., Ravishankar N., Subbanna G. N., Madras G ., Langmuir, 2004, 20, 2900— 2907
[32]	Lobo A., Moller T., Nagel M., Borchert H., Hickey S.G., Weller H., ,. J. Phys. Chem. B 2005, 109, 17422— 17428
[33]	Wang L., Wang W., Zhang W., Chen Y., Cao W., Shi H., Cao M., ,. Appl. Surf. Sci. 2018, 427, 553— 560
[34]	Berglund S. P., Rettie A. J., Hoang S., Mullins C. B., ,Phys. Chem. Chem. Phys., 2012, 14, 7065— 7075
[35]	Zhao X. M., Zhou S. W., Shen Q. M., Jiang L. P., Zhu J. J ., Analyst, 2012, 137, 3697— 3704
[36]	Tu W. W., Lu J., Dong Y. T., Lei J. P., Ju H. X., ,Anal. Chem., 2010, 82, 8711— 8716
[37]	Xin Y. M., Li Z. Z., Wu W. L., Fu B. H., Wu H. J., Zhang Z. H., ,Biosens. Bioelectron., 2017, 87, 396— 403
[38]	Zhao W. W., Xu J. J., Chen H. Y., ,Chem. Rev., 2014, 114, 7421— 7441
[39]	Zhuo K., Gu Y. S., Yan X. Q., Bai Z. M., Liu Y. C., ,. Biosens. Bioelectron. 2015, 64, 499— 504

基于PbS QDs/TiO₂ NPs构建新型谷胱甘肽光电化学传感器

Novel Glutathione Photoelectrochemical Sensor Based on PbS QDs/TiO₂ NPs ^†

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 13

参考文献 39

相关文章 15

编辑推荐

Metrics

本文评价

[1]	王君旸, 刘争, 张茜, 孙春燕, 李红霞. DNA银纳米簇在功能核酸荧光生物传感器中的应用[J]. 高等学校化学学报, 2022, 43(6): 20220010.
[2]	刘家琪, 李天保. BiVO₄/CuBi₂O₄薄膜光电极的制备及光电性能[J]. 高等学校化学学报, 2022, 43(4): 20220017.
[3]	唐定, 衷水平. Bi_1-_xFe_xVO₄薄膜光阳极的制备及光电化学性能[J]. 高等学校化学学报, 2021, 42(8): 2509.
[4]	徐梦祎, 黄雪雯, 李小杰, 魏玮, 刘晓亚. “串珠状”复合纳米组装体修饰丝网印刷电极构建的生物传感器[J]. 高等学校化学学报, 2021, 42(6): 1768.
[5]	王博东, 潘美辰, 卓颖. 二氧化硅纳米颗粒表面原位还原银纳米簇电化学发光传感界面的构建与分子识别[J]. 高等学校化学学报, 2021, 42(11): 3519.
[6]	张嘉懿, 丁臻尧, 王丹丹, 陈礼平, 封心建. 基于多孔金结构的三相界面酶电极的制备及高效电化学酶传感性能[J]. 高等学校化学学报, 2021, 42(10): 3167.
[7]	韩方杰, 代梦娇, 梁芷珊, 宋忠乾, 韩冬雪, 牛利. 光电化学技术应用于抗氧化分析的研究进展[J]. 高等学校化学学报, 2020, 41(4): 591.
[8]	凌云云,李莉,梁修蓉,夏云生. 基于金@二氧化锰纳米片超级纳米粒子从比色到单颗粒光谱检测谷胱甘肽[J]. 高等学校化学学报, 2020, 41(4): 623.
[9]	张怡萌, 张慧欣, 刘洋. 外泌体生物分析及其临床应用研究进展[J]. 高等学校化学学报, 2020, 41(11): 2306.
[10]	周羽婷, 汤玉娇, 邵爽, 戴诗岩, 程圭芳, 何品刚, 方禹之. 构象转换型传感器对汞、铅、锶离子的同时检测[J]. 高等学校化学学报, 2019, 40(8): 1621.
[11]	王春燕,蒋晓青,周泊. 基于Cu-TPA的电化学生物传感器对黄曲霉毒素B1的检测[J]. 高等学校化学学报, 2019, 40(11): 2301.
[12]	梁芷珊, 倪爽, 代梦娇, 韩方杰, 韩立鹏, 牛利, 韩冬雪. 基于g-C₃N₄/P25复合光电敏感体系的中草药抗氧化容量测定[J]. 高等学校化学学报, 2019, 40(10): 2081.
[13]	黄海平, 岳亚锋, 徐亮, 吕连连, 胡咏梅. 基于Dy₂(MoO₄)₃-AuNPs复合纳米材料的葡萄糖生物传感器[J]. 高等学校化学学报, 2017, 38(4): 554.
[14]	刘仁植, 李晓严. MoS₂中空纳米球的制备及在超灵敏microRNA电化学生物传感器中的应用[J]. 高等学校化学学报, 2017, 38(3): 383.
[15]	尹居鑫, 李祖宏, 牟颖, 吕绍武, 罗贵民. 具有谷胱甘肽过氧化物酶活力的含硒三肽[J]. 高等学校化学学报, 2016, 37(7): 1302.