高等学校化学学报 ›› 2017, Vol. 38 ›› Issue (9): 1536.doi: 10.7503/cjcu20170233

• 分析化学 • 上一篇    下一篇

以Au/Cu纳米棒为基底的肺腺癌组织的特征表面增强拉曼光谱研究

佟倜1(), 赵洋洋2, 付义林1, 单桂晔2   

  1. 1. 吉林大学第二医院, 长春130041
    2. 东北师范大学物理学院, 长春 130024
  • 收稿日期:2017-04-13 出版日期:2017-09-10 发布日期:2017-08-24
  • 作者简介:联系人简介: 佟 倜, 男, 博士, 教授, 主要从事肿瘤医学及肿瘤的光谱特征研究. E-mail: tti666@163.com
  • 基金资助:
    国家自然科学基金(批准号: 11740077, 11774048)资助

Special Surface Enhanced Raman Scattering on Lung Cancer Tissues Based on Au/Cu Nanorods Substrate

TONG Ti1*1,2(), ZHAO Yangyang2, FU Yilin1, SHAN Guiye2   

  1. 1. The Second Hospital of Jilin University, Changchun 130041, China
    2. School of Physics, Northeast Normal University, Changchun 130024, China
  • Received:2017-04-13 Online:2017-09-10 Published:2017-08-24
  • Supported by:
    † Supported by the National Natural Science Foundation of China(Nos.11740077, 11774048)

摘要:

通过金铜共混法制备了Au/Cu合金纳米棒, 研究了铜掺杂对金纳米棒等离子体共振吸收和结构的影响, 探究了Au/Cu合金纳米棒的等离子体共振拉曼增强效应. 以Au/Cu合金纳米棒为基底对肺腺癌组织和癌旁正常组织进行了表面增强拉曼光谱检测. 结果显示, 癌变组织具有比癌旁正常组织更强的拉曼信号峰, 位于1250, 1344, 1408, 1568, 1608和2560 cm-1附近的拉曼峰分别与蛋白质的Amide Ⅱ氨基化合物、 C—H弯曲振动、 核酸中CH3的对称变角振动、 蛋白质色氨酸惰性环振动、 蛋白质酰胺I谱带分子间反平行β-折叠的C—O健伸缩振动和蛋白质的巯基(S—H)伸缩振动有关, 2936 cm-1附近的拉曼峰为蛋白质CH2的对称伸缩振动和CH3的反对称伸缩振动共同作用产生. 以铜掺杂的金纳米棒为基底的表面增强拉曼光谱法有望成为检测肺癌组织的有效手段.

关键词: 金纳米棒, 铜离子掺杂, 表面增强拉曼光谱, 肺癌组织

Abstract:

Au/Cu nanorods were prepared by mixing method. The influence of doping copper ions on the plasmon resonance absorption and morphology of gold nanorods was investigated. The plasmon resonance Raman enhancement effect on Au/Cu alloy nanorods was detected. The Raman molecular vibration information of lung cancer tissues and corresponding non-tumor normal tissues were compared on Au/Cu alloy nanorods substrate. Surface enhanced Raman scattering(SERS) analysis of lung cancer tissues and corresponding non-tumor normal tissues demonstrate that the Raman peaks of cancer tissues are stronger compared to non-tumor normal tissues because the SERS signals of protein molecules in lung adenocarcinoma are influenced by their spatial conformation and molecular symmetry. The results suggest that tissue carcinogenesis increases the symmetry of the molecular structure and the Raman peaks near 1250, 1344, 1408, 1568, 1608 and 2560 cm-1 are relevant to amino-compounds of amide Ⅱ in protein, C—H bending vibration, symmetrical variable angle vibration of CH3 in nucleic acids, inert ring vibration of tryptophan in protein and C—O stretching vibration of amide Ⅰ band in protein with intermolecular antiparallel β-fold and sulfhydryl(S—H) stretching vibration of proteins, respectively. The Raman peak near 2936 cm-1 is generated by the joint action of the symmetric stretching vibration of CH2 and the antisymmetric stretching vibration of CH3 in protein. Herein, SERS based on Au/Cu alloy nanorods as substrate is promising to be an effective method for the detection of lung cancer.

Key words: Au nanorods, Copper ion doping, Surface enhanced Raman spectrum, Lung cancer tissue

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