聚多巴胺包覆的金纳米棒用于大鼠下颌下腺造影成像

doi:10.7503/cjcu20180362

高等学校化学学报 ›› 2019, Vol. 40 ›› Issue (1): 166.doi: 10.7503/cjcu20180362

聚多巴胺包覆的金纳米棒用于大鼠下颌下腺造影成像

宋子琦, 公柏娟, 王璐, 冯婧, 王博, 闫弘静, 李志民(), 孙宏晨

吉林大学口腔医院, 长春 130021

收稿日期:2018-05-16 出版日期:2019-01-10 发布日期:2018-12-12
作者简介:
联系人简介: 李志民, 男, 博士, 副教授, 主要从事口腔颌面医学影像诊断研究. E-mail: zhimin@jlu.edu.cn
基金资助:
吉林省科技发展计划项目(批准号: 20170414047GH)和吉林大学高层次科技创新团队建设项目(批准号: 2017TD-11)资助.

Polydopamine-coated Gold Nanorods for Rat Submandibular Gland Radiography^†

SONG Ziqi, GONG Baijuan, WANG Lu, FENG Jing, WANG Bo, YAN Hongjing, LI Zhimin^*(), SUN Hongchen

School and Hospital of Stomatology, Jilin University, Changchun 130021, China

Received:2018-05-16 Online:2019-01-10 Published:2018-12-12
Contact: LI Zhimin E-mail:zhimin@jlu.edu.cn
Supported by:
† Supported by the Jilin Province Science and Technology Development Project, China(No.20170414047GH)and the Jilin University Science and Technology Innovative Research Team Project, China(No.2017TD-11)

摘要/Abstract

摘要：

用生物相容性好且毒性低的聚多巴胺对金纳米棒进行表面包覆, 利用其造影增强的功能, 将聚多巴胺包覆的金纳米棒应用于大鼠下颌下腺导管, 实现了下颌下腺造影成像.

关键词: 金纳米棒, 计算机体层扫描, 下颌下腺, 造影成像

Abstract:

We demonstrate the application of polydopamine-coated gold nanorods in the CT imaging of rat submandibular gland. Biocompatible polydopamine was coated on the surface of gold nanorods to lower the toxicity. CT value rises with the increase of the concentration of polydopamine-coated gold nanorods in vitro, which indicates the high sensitivity to X-ray. The polydopamine-coated gold nanorods are further applied to rat submandibular gland in vivo and radiographs are obtained. H&E staining of heart, liver, spleen, lungand kidney show no obvious inflammation for the rats.

Key words: Gold nanorods, Computerized tomography, Submandibular gland, Radiography imaging

中图分类号:

O631

TrendMD:

宋子琦, 公柏娟, 王璐, 冯婧, 王博, 闫弘静, 李志民, 孙宏晨. 聚多巴胺包覆的金纳米棒用于大鼠下颌下腺造影成像. 高等学校化学学报, 2019, 40(1): 166.

SONG Ziqi,GONG Baijuan,WANG Lu,FENG Jing,WANG Bo,YAN Hongjing,LI Zhimin,SUN Hongchen. Polydopamine-coated Gold Nanorods for Rat Submandibular Gland Radiography^†. Chem. J. Chinese Universities, 2019, 40(1): 166.

图/表 7

Fig.1 TEM images(A, B) and UV-Vis-NIR absorption spectra(C, D) of gold nanorods(A, C) and polydopamine coated gold nanorods(B, D)

Fig.2 FTIR spectra of CTAB(a), SDS(b), DA(c), AuNRs(d) and AuNRs@PDA(e)

Fig.3 XRD(A) and XPS(B) spectra of gold nanorods

Fig.4 In vitro contrast enhanced CT imaging of AuNRs@PDA(A1—A6) and CT values of AuNRs@PDA with different concentrations(B)Concentration of AuNRs@PDA/(mg·mL-1): (A1) 0; (A2) 0.25; (A3) 0.5; (A4) 1; (A5) 2; (A6) 3.

Fig.5 Cytotoxicities of gold nanorods and polydopamine coated gold nanorods(A) and CTAB, SDS and DA(B) with concentrations

Fig.6 In vivo submandibular gland imaging with AuNRs@PDA of different concentrationsConcentration of AuNRs@PDA/(mg·mL-1): (A) 0; (B) 0.25; (C) 0.5; (D) 1; (E) 2; (F) 3.In these figures, the arrow points to the position of the submandibular gland.

Fig.7 Histological evaluation of AuNRs@PDA toxicity in vivo in rat submandibular gland(A, A'), heart(B, B'), liver(C, C'), spleen(D, D'), lung(E, E') and kidney(F, F') by H&E staining method(A—F) Control; (A'—F') experiment.

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聚多巴胺包覆的金纳米棒用于大鼠下颌下腺造影成像

Polydopamine-coated Gold Nanorods for Rat Submandibular Gland Radiography^†

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聚多巴胺包覆的金纳米棒用于大鼠下颌下腺造影成像

Polydopamine-coated Gold Nanorods for Rat Submandibular Gland Radiography†

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Polydopamine-coated Gold Nanorods for Rat Submandibular Gland Radiography^†