高等学校化学学报 ›› 2017, Vol. 38 ›› Issue (6): 1040-1045.doi: 10.7503/cjcu20160710

• 分析化学 • 上一篇    下一篇

表面增强拉曼光谱对西地那非类药物的快速检测

王红梅1, 李玲玲1, 陈海滨1, 曾勇明2,3, 陈宏炬4, 陈启振2,3, 林惠真4, 田中群4, 刘国坤2()   

  1. 1. 厦门市食品药品质量检验研究院, 厦门 361012
    2. 厦门大学环境与生态学院, 近海海洋环境科学国家重点实验室, 厦门 361102
    3. 厦门市普识纳米科技有限公司, 厦门361005
    4. 厦门大学化学化工学院, 厦门 361005
  • 收稿日期:2016-10-11 出版日期:2017-06-10 发布日期:2017-05-23
  • 作者简介:联系人简介: 刘国坤, 男, 博士, 副教授, 主要从事表面增强拉曼光谱在食品安全和环境监测中的应用研究. E-mail: guokunliu@xmu.edu.cn
  • 基金资助:
    国家自然科学基金(批准号: 21473140)、 福建省高校产学合作项目(批准号: 2016Y4012)和中央高校基本科研业务费专项资金(批准号: 2072016011)资助.

Application of Surface-enhanced Raman Spectroscopy in the Fast Screening of Phosphodiesterase Type 5 Inhibitors

WANG Hongmei1, LI Lingling1, CHEN Haibin1, ZENG Yongming2,3, CHEN Hongju4, CHEN Qizhen2,3, LIN Huizhen4, TIAN Zhongqun4, LIU Guokun2,*()   

  1. 1. Xiamen Institute for Food and Drug Quality Control, Xiamen 361012, China
    2. College of the Environment & Ecology, State Key Laboratory of Marine Environmental Science,Xiamen University, Xiamen 361102, China;
    3. PERSer Nanotechnology Ltd. Co., Xiamen 361005, China
    4. College of Chemistry & Chemical Engineering, Xiamen University, Xiamen 361005, China;
  • Received:2016-10-11 Online:2017-06-10 Published:2017-05-23
  • Contact: LIU Guokun E-mail:guokunliu@xmu.edu.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(No.21473140), the University-Industry Cooperation Foundation of Fujian Province, China(No.2016Y4012) and the Fundamental Research Funds for the Central Universities, China(No.2072016011).

摘要:

采用表面增强拉曼光谱(SERS)技术并结合简单的前处理流程, 对保健品样品中11种西地那非类药物进行了非定向快速筛查研究. 结果表明, 11种西地那非类药物可根据结构分为5类, 类别之间SERS谱图差异显著; 类别内SERS谱图具有共性特征, 特征峰相对强度差异明显. 实际样品的检测中, 西地那非类药物的最低检出浓度约为0.05 mg/kg; 前处理和测试的总时长约为3~5 min, 且与检测目标物和样品无关. 本方法高灵敏度、 快速和非定向检测的设计理念为快速检测保健品中违禁添加药物提供了新思路和新方法.

关键词: 表面增强拉曼光谱, 西地那非类药物, 非定向快速检测, 高灵敏度

Abstract: Aim

ing at the absence of a fast and effective screening technique facing the prevalence of the illegal adulterant of Phosphodiesterase type 5(PDE-5) inhibitor in health products, a non-designated detection platform based on surface-enhanced Raman spectroscopy(SERS) was proposed. Integrated with a simple pretreatment process in 3—5 min, SERS measurement was carried out on those suspicious health product samples with the lowest detectable concentration at a level of 0.05 mg/kg. The advantages of short time and high sensitivity satisfy the urgent demand of the fast screening and on-site inspection of the authorities.

Key words: Surface-enhanced Raman spectroscopy, Phosphodiesterase type 5(PDE-5), Non-targeting quick screening, High sensitivity

中图分类号: