自交联共轭亚油酸囊泡的温敏膨胀行为

doi:10.7503/cjcu20150079

高等学校化学学报 ›› 2015, Vol. 36 ›› Issue (6): 1140.doi: 10.7503/cjcu20150079

自交联共轭亚油酸囊泡的温敏膨胀行为

戴基宁, 樊晔, 方云()

江南大学化学与材料工程学院, 食品胶体与生物技术教育部重点实验室, 无锡 214122

收稿日期:2015-01-23 出版日期:2015-06-10 发布日期:2015-04-14
作者简介:联系人简介: 方云, 男, 博士, 教授, 博士生导师, 主要从事胶体与界面化学研究. E-mail:yunfang@126.com
基金资助:
国家自然科学基金(批准号: 21276113)资助

Temperature-responsive Expansion of Self-crosslinked Ufasomes of Conjugated Linoleic Acid^†

DAI Jining, FAN Ye, FANG Yun^*()

Key Laboratory of Food colloids and Biotechnology, Ministry of Education,School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China

Received:2015-01-23 Online:2015-06-10 Published:2015-04-14
Contact: FANG Yun E-mail:yunfang@126.com
Supported by:
† Supported by the National Natural Science Foundation of China(No.21276113)

摘要/Abstract

摘要：

采用紫外辐照引发共轭亚油酸(CLA)囊泡内聚合反应, 获得自交联CLA囊泡. 用动态激光光散射(DLS)和冷冻透射电子显微镜(cryo-TEM)等观察自交联CLA囊泡的粒径和形貌变化, 结果表明, 自交联CLA囊泡的囊泡结构不仅不随温度变化解体, 而且表现出明显的温敏膨胀性. 自交联CLA囊泡体外释放5-氟尿嘧啶的实验表明其具有温度响应的缓释和控释特性.

关键词: 囊泡, 自交联共轭亚油酸, 形貌, 温敏膨胀行为, 体外释放

Abstract:

Self-crosslinked ufasomes of conjugated linoleic acid(SCLA ufasomes) were obtained from CLA ufasomes through intravesicular polymerization initiated by mild UV irradiation. Temperature-responsive size and morphology evolutions of the SCLA ufasomes were traced by dynamic light scattering(DLS) and cryo-transmission electron microscope(cryo-TEM), and it was found that not only the vesicular structure of the SCLA ufasomes was stable but also the vesicular volume was expansive hugely with increasing temperature. In vitro release of 5-Fluorouracil(5-FU) from the SCLA ufasomes was evaluated and the results demonstrated that the drug-loading SCLA ufasomes possessed temperature-responsive sustained and controlled release of 5-FU.

Key words: Ufasome, Self-crosslinked conjugated linoleic acid, Morphology, Temperature responsive expansion, In vitro release

中图分类号:

O648

TrendMD:

戴基宁, 樊晔, 方云. 自交联共轭亚油酸囊泡的温敏膨胀行为. 高等学校化学学报, 2015, 36(6): 1140.

DAI Jining, FAN Ye, FANG Yun. Temperature-responsive Expansion of Self-crosslinked Ufasomes of Conjugated Linoleic Acid^†. Chem. J. Chinese Universities, 2015, 36(6): 1140.

图/表 6

Fig.1 Effect of UV-irradiation time on UV spectra of SCLA ufasomespH=8.6; c=3 mmol/L. UV-irradiation time/h: a. 0; b. 0.25; c. 0.5; d. 1; e. 1.5; f. 2; g. 2.5.

Fig.2 Size distribution and morphology of CLA ufasomes and SCLA ufasomes(3 mmol/L, pH=8.6, 25 ℃) (A) Size(2Rh) distribution. a. CLA vesicle, b. self-crosslinked CLA ufasomes with 47% crosslinking degree; (B) cryo-TEM image of CLA ufasomes; (C) cryo-TEM image; (D) TEM image of SCLA ufasomes with 47% polymerization degree.

Fig.3 Size sensitive(A) and structure stable(B) of SCLA ufasomes(3 mmol/L, pH=8.6) at different temperatures (A) Rh/nm: a. 8.1; b. 9.2; c. 10.4; d. 12.1; e. 15.5; f. 16.4. (B) Temperature/℃: a. 25; b. 30; c. 35; d. 40; e. 45; f. 50.

Fig.4 Temperature-responsive expansion of SCLA ufasomes(3 mmol/L, pH=8.6) (A1—A3) Rh distribution; (B1—B3) cryo-TEM images; (C1—C3) assessment of wall thickness. (A1—C1) 25 ℃; (A2—C2) 37 ℃; (A3—C3) 50 ℃.

Fig.5 Release behavior of 5-FU in PBS buffer(A) Standard UV curve; (B) in vitro release curve. a. Free drug, 25 ℃;b. loaded drug, 42 ℃; c. loaded drug, 37 ℃; d. loaded drug, 25 ℃.

Table 1 Formations of in vitro release of 5-FU in PBS buffer

Release temperature/℃	R²				Weibull equation
Release temperature/℃	Zero-order		First-order	Higuchi	Weibull
25^*	0.9056	0.9569	0.9646	0.9892	ln[-ln(1-Q)]=1.24lnt-3.74
25	0.6709	0.8941	0.8093	0.9472	ln[-ln(1-Q)]=0.6401lnt-2.388
37	0.6478	0.8363	0.7951	0.9557	ln[-ln(1-Q)]=0.4877lnt-1.705
42	0.6852	0.9458	0.7549	0.9859	ln[-ln(1-Q)]=0.5748lnt-1.8426

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自交联共轭亚油酸囊泡的温敏膨胀行为

Temperature-responsive Expansion of Self-crosslinked Ufasomes of Conjugated Linoleic Acid^†

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自交联共轭亚油酸囊泡的温敏膨胀行为

Temperature-responsive Expansion of Self-crosslinked Ufasomes of Conjugated Linoleic Acid†

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Temperature-responsive Expansion of Self-crosslinked Ufasomes of Conjugated Linoleic Acid^†