两性离子自组装仿生表面的制备、 表征及抗黏附性能

doi:10.7503/cjcu20140160

摘要/Abstract

摘要：

设计与合成了磺酸甜菜碱型的两性离子化合物: N,N-二甲基氨甲酸乙酯基丙基三乙氧基硅烷磺酸内盐(SiNNS), 利用红外光谱(FTIR)和氢核磁共振波谱(¹H NMR)对其分子组成与结构进行了表征. 通过自组装技术将SiNNS分子构筑在玻璃基材表面, 形成了模拟细胞外层膜的仿生表面. 利用原子力显微镜(AFM)、 X光电子能谱(XPS)和接触角测量仪对表面的形貌特征、化学组成和润湿性进行了表征. 以空白玻璃为对照样品, 研究了这一表面的防雾性能和抗细菌黏附性能. 结果表明, 所制备的两性离子自组装仿生表面具有超亲水性和水下超疏油特性, 其水滴接触角为9.2°, 水下油滴接触角接近180°; 与对照样品相比, 两性离子自组装表面具有优异的防雾性与抗细菌黏附性.

关键词: 甜菜碱型两性离子, 自组装, 仿生表面, 超亲水, 抗细菌黏附性

Abstract:

A sulfobetaine zwitterionic compound, N,N-dimethylamino ethyl carbamate propyl triethoxysilane sulfonate(SiNNS), was designed and synthesized and its composition and molecular structure were characterized by means of FTIR and ¹H NMR spectroscopy. Furthermore, the biomimetic surface imitating the chemical features of cellular outer membrane were constructed via the self-assembly of SiNNS molecules on the hydroxylated glass surface. The morphology structure, chemical composition and wettability of the prepared biomimetic surface were characterized by atomic force microscopy(AFM), X-ray photoelectron spectroscopy(XPS) and contact angle measurement. The antifogging and antibacterial adhesion performances of the biomimetic surface were investigated using an untreated glass surface as a control sample. The results indicate that the zwitterionic self-assembled biomimetic surface can possess superhydrophilicity with a water contact angle of 9.2° and underwater superoleophobicity with an oil contact angle of 175.6°. Compared to the corresponding control sample, the zwitterionic self-assembled biomimetic surface can exhibit an excellent antifogging property and an obvious antibacterial adhesion performance.

Key words: Betaine-zwitterion, Self-assembly, Biomimetic surface, Superhydrophilicity, Antibacterial adhesion

中图分类号:

O647.4

TrendMD:

吴雅露, 李光吉, 刘云鸿, 陈达杨. 两性离子自组装仿生表面的制备、表征及抗黏附性能. 高等学校化学学报, 2014, 35(7): 1484.

WU Yalu, LI Guangji, LIU Yunhong, CHEN Dayang. Preparation, Characterization and Antibacterial Adhesion Performance of the Biomimetic Surfaces via Zwitterionic Self-assembly^†. Chem. J. Chinese Universities, 2014, 35(7): 1484.

图/表 9

Scheme 1 Synthesis routes of zwitterionic compound SiNNS

Fig.1 FTIR(A) and 1H NMR(B) spectra of SiNNS

Fig.2 AFM images and section plots for the SiNNS modified glass surfaces formed by soaking into 50 mmol/L SiNNS solution for different time Soaking time/min: (A) 0; (B) 30; (C) 60; (D) 90; (E) 120.

Fig.3 Schematic diagram of the formation of self-assembled membrane of zwitterionic compound iNNS on a hydroxylated glass surface

Fig.4 XPS spectra of the glass surface treated with 50 mmol/L SiNNS solution for 3 h (A) Full XPS spectrum of the treated surface; (B) the N1s region of the treated surface; (C) the S2p region of the treated surface.

Fig.5 Measurements of water droplet contact angles on different surfaces and oil droplet contact angles on Glass-SiNNS in different environments (A) Water droplet on Glass-B; (B) water droplet on Glass-OH; (C) water droplet on Glass-SiNNS; (D) oil droplet on Glass-SiNNS in air; (E) oil droplet on Glass-SiNNS under water.

Fig.6 Comparision of antifogging performance of Glass-SiNNS surface(left) and Glass-B surface(right)

Fig.7 Adhesion results of S. aureus(A, B) and E. Coli(C, D) on Glass-B surface(A, C) and Glass-SiNNS surface(B, D) contacted with bacterium suspension and cultured for different time (A1—D1) 6 h; (A2—D2) 12 h; (A3—D3) 36 h.

Fig.8 Variation of viable bacterial concentration with the culture time for bacterium suspension contacting with Glass-SiNNS surface (A)S. aureus; (B) E. coli.

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