基于正电荷和光热协同效应的新型半导体聚合物纳米抗菌材料

doi:10.7503/cjcu20190715

高等学校化学学报 ›› 2020, Vol. 41 ›› Issue (4): 670.doi: 10.7503/cjcu20190715

• 庆祝《高等学校化学学报》复刊40周年专栏 • 上一篇下一篇

基于正电荷和光热协同效应的新型半导体聚合物纳米抗菌材料

潘国勇¹,荔雅文¹,马立军¹,马宇帆¹,艾文婷¹,王振国¹,侯欣慧¹,戈里戈瑞·齐格亚诺夫^2,³,王卓^1,^*()

^1. 化工资源有效利用国家重点实验室, 北京软物质科学与工程高精尖创新中心, 北京化工大学化学学院, 北京 100029
^2. 乌拉尔联邦大学, 叶卡捷琳堡 620002, 俄罗斯联邦
^3. 普斯托夫斯基有机合成研究所, 俄罗斯科学院乌拉尔分院, 叶卡捷琳堡 620108, 俄罗斯联邦

收稿日期:2019-12-30 出版日期:2020-04-10 发布日期:2020-02-26
通讯作者: 王卓 E-mail:wangzhuo77@mail.buct.edu.cn
基金资助:
国家自然科学基金(81961138011, 21775010);北京市自然科学基金(7192106);中央高校基本科研业务费(K1901, XK1802-6)

New Semiconducting Polymer Nanoparticles for Antibacterial Agent by the Synergetic Effect of Positive Charge and Photothermal Conversion

PAN Guoyong¹,LI Yawen¹,MA Lijun¹,MA Yufan¹,AI Wenting¹,WANG Zhenguo¹,HOU Xinhui¹,Grigory V·Zyryanov^2,³,WANG Zhuo^1,^*()

^1. State Key Laboratory of Chemical Resource Engineering, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, College of Chemistry, Beijing University of Chemical Technology, Beijing 100029, China
^2. Ural Federal University Yekaterinburg 620002, Russian Federation
^3. Postovsky Institute of Organic Synthesis, Ural Branch of the Russian Academy of Sciences, Yekaterinburg 620108, Russian Federation

Received:2019-12-30 Online:2020-04-10 Published:2020-02-26
Contact: Zhuo WANG E-mail:wangzhuo77@mail.buct.edu.cn
Supported by:
This paper is supported by the Natural Science Foundation of China(81961138011, 21775010);the Beijing Natural Science Foundation, China(7192106);the Fundamental Research Funds for the Central Universities, China(K1901, XK1802-6)

摘要/Abstract

摘要：

由于抗生素的不当使用和细菌多药耐药的出现, 迫切需要开发新的抗菌剂. 本文制备了具有光热转换性能的正电荷半导体高分子材料及具有协同抗菌活性的半导体聚合物纳米粒子(SP-PPh₃ NPs). SP-PPh₃ NPs的光热转化效率为43.8%. 带正电荷的SP-PPh₃ NPs可以附着在细菌上, 有助于将热量有效传递给细菌. 在热和正电荷的协同作用下, SP-PPh₃ NPs对革兰氏阴性大肠杆菌(E. coli)和革兰氏阳性金黄色葡萄球菌(S. aureus)均具有抗菌活性, 其对二者的体外抑菌率分别为99.9%和98.6%. 此外, SP-PPh₃ NPs具有良好的生物相容性, 对小鼠的主要器官几乎无副作用. 对细菌感染的小鼠皮肤伤口用SP-PPh₃ NPs治疗12 d后, 伤口可以很好地愈合.

关键词: 半导体聚合物, 正电荷纳米颗粒, 光热转换, 抗菌

Abstract:

Because of the abuse of antibiotics and the emergence of bacterial resistance, the new antibacterial agents are required urgently. Herein, we prepared semiconducting polymer nanoparticles(SP-PPh₃ NPs) with synergistic antibacterial activity due to photothermal properties and positive charge. SP-PPh₃ NPs have broad-spectrum antibacterial properties against Gram-negative Escherichia coli(E. coli) and Gram-positive Staphylococcus aureus(S. aureus). The photothermal conversion efficiency of SP-PPh₃ NPs is 43.8%. Moreover, the positive charge of SP-PPh₃ NPs can adhere to bacteria, which is helpful to transmit heat to bacteria effectively. Under the synergistic effect of heat and positive charge, the antibacterial rates of E. coli and S. aureustreated with SP-PPh₃ NPs are 99.9% and 98.6% in vitro, respectively. In addition, SP-PPh₃ NPs have good biocompatibility and have almost no side effects on the major organs of mice. The bacteria-infected skin wounds on mice can completely heal after 12 d treated with SP-PPh₃ NPs.

Key words: Semiconducting polymer, Positively charged nanoparticles, Photothermal conversion, Antibacterial

中图分类号:

O633

TrendMD:

潘国勇,荔雅文,马立军,马宇帆,艾文婷,王振国,侯欣慧,戈里戈瑞·齐格亚诺夫,王卓. 基于正电荷和光热协同效应的新型半导体聚合物纳米抗菌材料. 高等学校化学学报, 2020, 41(4): 670.

PAN Guoyong,LI Yawen,MA Lijun,MA Yufan,AI Wenting,WANG Zhenguo,HOU Xinhui,Grigory V·Zyryanov,WANG Zhuo. New Semiconducting Polymer Nanoparticles for Antibacterial Agent by the Synergetic Effect of Positive Charge and Photothermal Conversion. Chem. J. Chinese Universities, 2020, 41(4): 670.

图/表 7

Fig.1 Synthetic route of SP-PPh3

Fig.2 1H NMR(A, B) and FTIR(C, D) spectra of SP-Br(A) and SP-PPh3(B) (D) is the amplification of the marked part in (C).

Fig.3 Characterization and photothermal activity of materials(A) UV-Vis absorption spectra of SP-PPh3 and SP-PPh3 NPs; (B) DLS and TEM image(inset) of SP-PPh3 NPs; (C) temperature elevation of SP-PPh3 NPs aqueous solution(10, 15, 20, 30 μg/mL) and water under 808 nm laser(1.0 W/cm2); (D) temperature change of SP-PPh3 NPs aqueous solution(20 μg/mL) under irradiation of 808 nm laser(1.0 W/cm2) and turn off laser follow by natural cooling; (E) time-dependent natural logarithm of the temperature of SP-PPh3 NPs during the natural cooling after 16 min; (F) cyclic heating curve of SP-PPh3 NPs(20 μg/mL) under laser irradiation for five circles.

Fig.4 IC50 of SP-PPh3 NPs for E. coli(A) and S. aureus(B)

Fig.5 In vitro antibacterial activity evaluation and SEM images(A) Photographs of bacterial colony formation in the presence of E. coli(A1—A4) and S. aureus(A5—A8). (A1) PBS; (A2) laser; (A3) SP-PPh3 NPs; (A4) SP-PPh3 NPs+laser; (A5) PBS; (A6) laser; (A7) SP-PPh3 NPs; (A8) SP-PPh3 NPs+laser. (B) The relative bacterial viability of the control group(a), the laser group(b), the SP-PPh3 NPs group(c), the SP-PPh3 NPs+laser group(d). The concentration of SP-PPh3 NPs is 20 μg/mL and 808 nm laser power is 1.0 W/cm2. (C) SEM images of E. coli(C1—C3) and S. aureus(C4—C6) contacting with control(C1, C4), 20 μg/mL SP-PPh3 NPs(C2, C5), SP-PPh3 NPs+laser(C3, C6). The 808 nm laser power is 1.0 W/cm2.

Fig.6 Cytotoxicity and mice model experiments(A) Photographs of wounds in mice treated with PBS(control), SP-PPh3 NPs and SP-PPh3 NPs+laser on days 0, 3, 6, 9, 12; (B) IR thermal images of the wound treated without SP-PPh3 NPs and with SP-PPh3 NPs respectively under the laser irradiation(808 nm, 1.5 W/cm2); (C) cytotoxicity of SP-PPh3 NPs(10, 20, 30, 40, 50 μg/mL); (D) wounds area value on day 0, 3, 6, 9, and 12 during antibacterial therapy; (E) the body mass of mice treated with PBS(control), SP-PPh3 NPs and SP-PPh3 NPs+laser; (F) analysis of liver function in mice after 12 d by blood, including alanine aminotransferase(a, U/L), aspartate aminotransferase(b, U/L), alkaline phosphatase(c, U/L), total protein(d, g/L), albumin(e, g/L).

Fig.7 H&E staining analysis of skin and major organs(A) H & E staining slices of wound skin tissue on days 3, 6, 9, and 12; (B) mouse organ H & E staining sections(heart, liver, spleen, lung and kidney) of the control group and the SP-PPh3 NPs treated group.

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基于正电荷和光热协同效应的新型半导体聚合物纳米抗菌材料

New Semiconducting Polymer Nanoparticles for Antibacterial Agent by the Synergetic Effect of Positive Charge and Photothermal Conversion

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