海藻酸壳聚糖可塑性支架材料的制备及表征

doi:10.7503/cjcu20170565

摘要/Abstract

摘要：

利用海藻酸钠和壳聚糖2种原料, 采用阴阳离子静电复合原理, 通过滴注法层层自组装成可搭载药物的缓释微球, 再按一定比例与海藻酸钠-壳聚糖溶液混合制成缓释微球型支架材料, 将缓释微球结构嵌入疏松多孔海绵状结构中. 研究了缓释微球的组分比对缓释微球型支架材料的孔隙率、收缩率、亲水性及降解性能的影响; 扫描电子显微镜照片显示, 微球结构相对完整, 多孔海绵状结构孔径为140~200 μm; 支架浸出液细胞毒性检测实验组对照组未见差异. 缓释微球体积所占比例即组分比为10%的缓释微球型支架材料孔隙率最高为68.2%~70.8%, 亲水性最好, 收缩率最低为4.4%~5.2%; 支架降解速率随缓释微球组分比升高而减慢, 组分比为20%的缓释微球型支架材料综合性能更优; 缓释微球型支架材料冻干成型前为液态, 具有良好可塑性. 缓释微球型支架材料为缓释系统与多孔支架材料有机结合提供了新思路.

关键词: 组织工程支架, 海藻酸钠, 壳聚糖, 缓释微球

Abstract:

Based on anion and cation electrostatic load principle, using sodium alginate and chitosan as raw materials, sustained-release microspheres were obtained by the drip method and layer-by-layer self-assembly. The microspheres were then put into a certain percentage of sodium alginate-chitosan solution, and a new kind of scaffold with microspheres embedded in the spongy structure called sustained-release microsphere-type scaffolds was obtained. The influences of the component ratio of the sustained-release microspheres on the porosity, contraction, hydrophilicity and degradation properties of the sustained-release microsphere-type scaffolds were investigated. The pore size was between 140 and 200 μm analyzed by scanning electron microscopy(SEM). There was no difference in the cytotoxicity text of the scaffold’s leachate between the experimental group and the control group. When the component ratio of microspheres was 10%, the scaffolds had the highest porosity ratio(68.2%—70.8%), the lowest contraction percentage(4.4%—5.2%) and the best hydrophilicity. The less microspheres the scaffolds had, the faster the degradation rate was. For preventing the degradation rate too fast and carrying more medicines, the scaffolds, whose component ratio of microspheres was 20%, are much better. The microsphere-type scaffold material has good plasticity before freeze-drying. The microsphere-type scaffolds provided an example of the combination with sustained-release systems and existing porous scaffold materials.

Key words: Tissue engineering scaffold, Sodium alginate, Chitosan, Sustained-release microsphere

中图分类号:

O636

TrendMD:

刘志辉, 邱添源, 杜留熠, 杨军星, 柳康, 王博蔚. 海藻酸壳聚糖可塑性支架材料的制备及表征. 高等学校化学学报, 2018, 39(5): 1105.

LIU Zhihui,QIU Tianyuan,DU Liuyi,YANG Junxing,LIU Kang,WANG Bowei. Preparation and Characterization of Chitosan Alginate Plastic Scaffolds^†. Chem. J. Chinese Universities, 2018, 39(5): 1105.

图/表 10

Fig.1 FTIR spectra of scaffolds Note:a. SA; b. CS; c. CA-CS-SA-CS microspheres; d. scaffolds without microspheres; e. scaffolds with microspheres.

Fig.2 Optical photos of frontal view(A) and side view(B) of the scaffolds

Fig.3 SEM images of the scaffolds Note:(A) Microsphere; (B) edge of left microsphere and right spongy organization; (C) surface of spongy organization; (D) internal structure of spongy organization.

Fig.4 Porosity ratio of scaffolds with different component ratio of microspleres

Fig.5 Contraction percentage of scaffolds with different component ratio of microspleres

Fig.6 Swelling ratio of scaffolds with different component ratio of microspheres

Fig.7 Degradation of scaffolds with different component ratio of microspheres

Fig.8 Degradation of scaffolds with 30% microspheres in different time Note:Time/d:(A) 1; (B) 5; (C) 10; (D) 15; (E) 20.

Fig.9 Modulus of scaffolds with different component ratio of microspheres

Fig.10 Cytotoxicity test by compare growth of the cells under the medium with(a) and without(b) leachate of scaffolds

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