基于多巴胺的黏合水凝胶的制备及表征

doi:10.7503/cjcu20160443

摘要/Abstract

摘要：

以1-(3-二甲氨基丙基)-3-乙基碳二亚胺/N-羟基琥珀酰亚胺(EDC/NHS)为催化剂, 将多巴胺通过酰胺化反应连接到氧化海藻酸钠上, 制得含邻苯二酚结构的氧化海藻酸钠(COA), 将COA与胶原(COL)复合, 制得具有黏合性能的COA-COL水凝胶, 通过紫外-可见光谱(UV-Vis)、傅里叶变换红外光谱(FTIR)和核磁共振波谱(¹H NMR)方法表征了COA的结构, 用扫描电子显微镜、体外膨胀降解、万能电子拉力机和噻唑蓝(MTT)法分别表征了COA-COL水凝胶的形貌结构、平衡溶胀率、降解率、黏合强度和细胞毒性. 研究结果表明, 多巴胺已引入到氧化海藻酸钠上, 取代度分别为12.5%, 18%和22%; COA-COL水凝胶的内部形成了典型的海绵体多孔网状结构, 平衡溶胀率随着多巴胺取代度的提高先减小后增大, 降解率随着取代度的提高依次减小, COA-COL水凝胶的黏合强度从未经多巴胺改性时的(20.43±4.21) kPa提高到多巴胺取代度为22%时的(29.91±6.10) kPa, 而且具有良好的生物相容性.

关键词: 多巴胺, 氧化海藻酸钠, 胶原, 水凝胶, 黏合剂

Abstract:

In order to replace conventional sutures in wound closing applications, a favorable bioadhesive that has strong bonding strength, good biocompatibility and appropriate biodegradability is extremely needed. In this study, periodate oxidized alginate was modified by dopamine to obtain catechol-based oxidized alginate(COA) using 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide/N-hydroxysuccinimide(EDC/NHS) as catalyst. Then COA was crosslinked with collagen to further obtain a novel COA-collagen bioadhesive hydrogel. The microstructure of COA was characterized by ultraviolet-visible(UV-Vis), Fourier transform infrared spectrophotometer(FTIR) and proton nuclear magnetic resonance(¹H NMR) spectroscopy. Meanwhile, the structure, equilibrium swelling ratio, degradation rate, bonding strength and cytotoxicity of the hydrogel were also evaluated. The results showed that the grafting-copolymerization of dopamine to oxidized alginate happened successfully and the degree of the substitution(DS) of dopamine was 12.5%, 18% and 22%, respectively. The hydrogels exhibited typical porous network. The equilibrium swelling ratio of the hydrogels decreased firstly and increased with the substitution ratio of dopamine increasing at last. The degradation rate of the hydrogels became lower with the increase of dopamine substitution ratio. The bonding strength of the hydrogel increased from (20.43±4.21) kPa to (29.91±6.10) kPa after modified with dopamine. Moreover, the results obtained in the methyltetrazolium(MTT) study showed that no extra cytotoxicity, which was of importance to develop a kind of biomaterials. In conclusion, the results suggest that the COA-COL bioadhesive hydrogel can potentially be a promising alternative for use in wound closing applications.

Key words: Dopamine, Periodate oxidized alginate, Collagen, Hydrogel, Bioadhesion

中图分类号:

O636.9

TrendMD:

崔国廉, 但年华, 但卫华. 基于多巴胺的黏合水凝胶的制备及表征. 高等学校化学学报, 2017, 38(2): 318.

CUI Guolian, DAN Nianhua, DAN Weihua. Preparation and Characterization of Novel Dopamine-based Bioadhesive Hydrogels^†. Chem. J. Chinese Universities, 2017, 38(2): 318.

图/表 8

Fig.1 UV-Vis spectra of dopamine(a), COA-0(b), COA-12.5%(c), COA-18%(d) and COA-22%(e)

Fig.2 FTIR spectra of dopamine(a), alginate(b), COA-0(c), COA-12.5%(d), COA-18%(e) and COA-22%(f)

Fig.3 1H NMR spectra of COA-0(a), COA-12.5%(b), COA-18%(c) and COA-22%(d)

Fig.4 SEM images of COA-COL-0(A), COA-COL-12.5%(B), COA-COL-18%(C) and COA-COL-22%(D)

Fig.5 Equilibrium swelling ratio(A) and degradation(B) of COA-COL hydrogelsa. COA-COL-0; b. COA-COL-12.5%; c. COA-COL-18%; d. COA-COL-22%.

Fig.6 Lap shear strength of COA-COL hydrogelsa. Alg-dopa-col-20%; b. COA-COL-0; c. COA-COL-12.5%; d. COA-COL-18%; e. COA-COL-22%.

Scheme 1 Schematic illustration of the application of COA-COL bioadhesive hydrogels

Fig.7 Cell proliferation test by MTT assay on the 1st, 3rd and 5th day after seedinga. COA-COL-0; b. COA-COL-12.5%; c. COA-COL-18%; d. COA-COL-22%.

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