牛血清白蛋白在医用聚氨酯弹性体表面的吸附

高等学校化学学报 ›› 2011, Vol. 32 ›› Issue (11): 2691.

牛血清白蛋白在医用聚氨酯弹性体表面的吸附

尹红根¹, 侯逢文¹, 李艺¹, 程镕时²

1. 苏州大学材料与化学化工学部, 苏州 215123;
2. 南京大学化学化工学院, 南京 210093

收稿日期:2010-11-11 修回日期:2011-05-05 出版日期:2011-11-10 发布日期:2011-10-14
通讯作者: 李艺 E-mail:liyi@suda.edu.cn
基金资助:
江苏省高校自然科学基础研究计划(批准号: 08KJB430014)和国家自然科学基金(批准号: 21074086)资助.

Adsorption of Bovine Serum Albumin on the Surface of Medical Polyurethane Elastomer

YIN Hong-Gen¹, HOU Feng-Wen¹, LI Yi^1*, CHENG Rong-Shi²

1. College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China;
2. School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China

Received:2010-11-11 Revised:2011-05-05 Online:2011-11-10 Published:2011-10-14
Contact: LI Yi E-mail:liyi@suda.edu.cn
Supported by:
江苏省高校自然科学基础研究计划(批准号: 08KJB430014)和国家自然科学基金(批准号: 21074086)资助.

摘要/Abstract

摘要： 制备了具有较好机械性能的异佛尔酮二异氰酸酯(IPDI)型聚氨酯弹性体胶片, 并进行表面肝素化处理, 得到抗凝血医用导管材料. 将聚氨酯胶片浸泡在牛血清白蛋白(BSA)水溶液中, 利用稀溶液黏度法研究了牛血清白蛋白在聚氨酯胶片表面的动态吸附情况, 并采用界面校正黏度方程计算溶液浓度变化. 研究发现, 牛血清白蛋白分子能迅速吸附到聚氨酯胶片表面, 但达到吸附平衡需要较长时间. 牛血清白蛋白在聚氨酯表面吸附后的溶液中分子构象发生变化.

关键词: 聚氨酯弹性体, 牛血清白蛋白, 吸附, 黏度

Abstract: A special isophorone diisocyanate(IPDI) type polyurethane(PU) elastomer with good mechanical property was prepared. Heparin was grafted on the surface of this PU elastomer and a PU-Hep elastomer was obtained. The dynamic adsorption of bovine serum albumin(BSA) on PU elastomers was investigated by immersing the PU and PU-Hep elastomers in dilute BSA aqueous solution and measuring the viscosity change of the solution. The results show that BSA macromolecules quickly accumulate on the surface of PU and PU-Hep elastomers, however, it needs a long time to reach the adsorption equilibrium. The conformational chang of BSA macromolecules happens in bulk solution after adsorption on the PU and PU\|Hep elastomers.

Key words: Polyurethane elastomer; , Bovine serum albumin(BSA); , Adsorption, Viscosity

TrendMD:

尹红根侯逢文李艺程镕时. 牛血清白蛋白在医用聚氨酯弹性体表面的吸附. 高等学校化学学报, 2011, 32(11): 2691.

YIN Hong-Gen, HOU Feng-Wen, LI Yi*, CHENG Rong-Shi. Adsorption of Bovine Serum Albumin on the Surface of Medical Polyurethane Elastomer. Chem. J. Chinese Universities, 2011, 32(11): 2691.

参考文献

参考文献 [1] Bernacca G M, Gulbransen M J, Wilkinson R, et al. In vitro blood compatibility of surface-modified polyurethanes. Biomaterials[J], 1998,19: l151-1165 [2] Blomberg E, Claesson P M. Surface coated with protein layers: a surface force and ESCA study. Biomaterials[J], 1998, 19: 371-386． [3] Khan I, Smith N, Jones E, et a1．Analysis and evaluation of a biomedical polycarbonate urathane tested in an in vitro study and an ovine arthroplasty mode1．Part I：materials selection and evaluation．Biomaterials[J], 2005, 26(5): 621-631 [4] Jiang Y, Rongbing B, Ling T, et a1. Blood compatibility of polyurethane surface grafted copolymerization with sulfobetaine monomer．Co11oids Surf B Biointerfaces[J], 2004,36(1): 27-33 [5] 胡国栋．聚氨酯的血液相容性评价, 国外医学: 生物医学工程分册[J], 2002,25(6): 271-273 [6] 许乾慰,肖世英. 脂肪族聚氨酯弹性体肝素化的血液相容性研究，中国组织工程研究与临床康复[J], 2007, 11(13):2415-2419 [7] 王琴梅,潘仕蒙,郑振声. 肝素化聚氨酯表面血小板粘附研究, 北京生物医学工程[J], 1997, l6(l):44-48 [8] Wilson D F (editor)．Techniques of Biocompatibility Testing[M]．Florida: CRC Press Inc,1984 [9] Chen H, Zhang Y, Li D, et al. Protein Adsorption on Lysine-PEG immobilized PU Surfaces. 1st Asian Biomaterials Congress, December, 2007, Tsukuba, Japan [10] Cai J, Bo S, Qin W, Yan X, Cheng R. Appl Chem. [J], 2001, 18(5): 377 -380 [11] Cheng R. Effect of adsorption on the viscosity of dilute polymer solution.Chinese Journal of Polymer Science[J], 1999,17(1): 27-35 [12] Pan Y, Cheng R. A novel interpretation of concentration dependence of viscosity of dilute polymer solution. Chineses Journal of Polymer Science[J], 2000, 18(1): 57-67 [13] 朱志博, 许宏, 路新卫, 蔡玉春. 医用聚氨酯弹性体的合成及性能研究, 第一军医大学分校学报[J], 2004, 27(1): 24-25 [14] Li Y, Cheng R. A viscometric study for gelatin in dilute aqueous solution. J.Polym.Sci. Part B: Polym. Phys.[J], 2006, 44, 1804-1812 [15] Kazuhiko l, Hisuko H, Nobuo N．An tithrombotics and antiplatelet agent in vitro blood compatibility of modified polyurethanes．Biomaterials[J], 1995, 16(11):873-879． [16] Flory P J. Principle of Polymer Chemistry[M]. Cornell University:New York, 1953. [17] Uversky V N. Narizhneva N V. Ivanova T V. Biochemistry[J], 1997, 36, 13638