高等学校化学学报

高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (8): 1506.

• 研究论文 • 上一篇    下一篇

芳香胺的碱坝聚焦-毛细管电泳分析

王晓瑜1, 陈义1,2   

  1. 1. 中国科学院化学研究所, 活体分析化学重点实验室, 北京100190;
    2. 北京分子科学国家实验室, 北京100190
  • 收稿日期:2010-03-08 出版日期:2010-08-10 发布日期:2010-08-10
  • 通讯作者: 陈义, 男, 博士, 研究员, 博士生导师, 主要从事毛细管/芯片电泳及微量生物活性物质分析. E-mail: chenyi@iccas.ac.cn
  • 基金资助:

    国家自然科学基金(批准号： 90717120)资助.

Online Base Barrage Focusing and Capillary Electrophoresis of Aromatic Amines

WANG Xiao-Yu1, CHEN Yi1,2*   

  1. 1. Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China;
    2. Beijing National Laboratory of Molecular Science, Beijing 100190, China
  • Received:2010-03-08 Online:2010-08-10 Published:2010-08-10
  • Contact: CHEN Yi. E-mail: chenyi@iccas.ac.cn
  • Supported by:

    国家自然科学基金(批准号： 90717120)资助.

PDF (PC)

被引次数

18

摘要： 建立了碱坝聚焦-毛细管电泳方法, 实现了一些芳香胺的高灵敏检测. 该方法操作简单, 只需在大体积进样前先泵入一段NaOH溶液. 当进样5 cm长时, 间苯二胺、邻苯二胺及三聚氰胺的检出限可分别降至0.1, 0.05和0.05 μmol/L, 灵敏度比常规进样提高了100倍. 在2个量级浓度范围内, 各分析物峰面积与浓度具有良好的线性关系, 线性相关系数大于0.999. 该法可用于分析染发剂样品.

关键词: 在线碱坝聚焦, 芳香胺, 染发剂, 毛细管电泳

Abstract: A base barrage focusing approach online-coupled to capillary electrophoresis(CE) was developed and used for the analysis trace aromatic amines. To realize online focusing, a plug of 200 mmol/L NaOH was introduced in front of a sample zone just before electrophoresis. This method is facile and can be conducted on any CE system. After some optimization of the base barrage length and concentration, the method could be applied to the assay of m-phenylenediamine, o-phenylenediamine and melamine, with limits of detection down to 0.10 μmol/L m-phenylenediamine, 0.05 μmol/L o-phenylenediamine and 0.05 μmol/L melamine respectively, producing about 100-fold increase of sensitivity compared with the common method. Linear relationship was found between the peak area and sample concentration over two grades of magnitude, with linear correlation coefficients better than 0.999. As a validation, the method has also been applied to the analysis of a commercial hair dye, with the recovery between 94% and 97%.

Key words: Base barrage focusing, Aromatic amines, Hair dye, Capillary electrophoresis

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