高等学校化学学报

高等学校化学学报 ›› 2009, Vol. 30 ›› Issue (5): 1018.

• 研究论文 • 上一篇    下一篇

聚乳酸接枝改性纳米生物玻璃/PLGA复合材料的制备、表面性质及生物活性

董树君1,2, 于婷2, 魏俊超2, 景遐斌2, 周延民1, 章培标2, 陈学思2   

    1. 吉林大学口腔医学院, 长春 130041;
    2. 中国科学院长春应用化学研究所, 高分子物理与化学国家重点实验室, 长春 130022
  • 收稿日期:2009-01-13 出版日期:2009-05-10 发布日期:2009-05-10
  • 通讯作者: 周延民, 男, 教授, 博士生导师, 主要从事口腔种植学科研和临床工作, E-mail: zhouym@jlu.edu.cn; 章培标, 男, 博士, 副研究员, 主要从事组织工程和生物医用高分子研究, E-mail: zhangpb@ciac.jl.cn
  • 基金资助:

    国家自然科学基金(批准号: 50673090, 50733003)和国家“八六三”计划(批准号: 2007AA03Z320)资助.

Preparation, Surface Properties and Biological Activity of the Composite of Poly(lactide-co-glycolide) and Bioglass Nanoparticles Surface Grafted with Poly(L-lactide)

DONG Shu-Jun1,2, YU Ting2, WEI Jun-Chao2, JING Xia-Bin2, ZHOU Yan-Min1*, ZHANG Pei-Biao2*, CHEN Xue-Si2   

    1. Stomatological School of Jilin University, Changchun 130041,China;
    2. State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
  • Received:2009-01-13 Online:2009-05-10 Published:2009-05-10
  • Contact: ZHOU Yan-Min, E-mail: zhouym@jlu.edu.cn; ZHANG Pei-Biao, E-mail: zhangpb@ciac.jl.cn

PDF (PC)

被引次数

13

摘要：

以丙交酯开环聚合原位接枝改性的纳米生物玻璃(PLLA-g-BG)与聚丙交酯-乙交酯(PLGA)复合材料为研究对象, 采用TGA, ESEM和EDX分析其接枝率, 粒子分散性和表面元素分布, 通过将兔成骨细胞种植于材料膜表面进行体外培养, 采用荧光染色法、NIH Image J图像分析软件、MTT法和流式细胞术等手段检测细胞在材料表面的平均黏附数量、扩展面积比、增殖能力和细胞周期的变化, 综合评价新型改性纳米复合材料的生物相容性和生物活性. 结果表明, 聚乳酸表面接枝改性可明显改善纳米生物玻璃粒子的团聚; PLGA中掺入一定比例的改性PLLA-g-BG可明显促进兔成骨细胞的黏附、扩展与增殖; 改性纳米生物玻璃的应用可提高生物可降解聚酯材料的生物相容性和生物活性.

关键词: 纳米生物玻璃, 表面改性, 聚丙交酯-乙交酯(PLGA), 复合材料, 生物活性

Abstract:

SiO2-CaO-P2O5 gel bioglass(BG) nanoparticles with the diameter of 40 nm were synthesized by sol-gel approach. The surface of BG nanoparticles was grafted through the ring-open polymerization of the L-lactide to yield poly(L-lactide)(PLLA) grafted gel particle(PLLA-g-BG). The PLLA-g-BG was further blended with poly(lactide-co-glycolide)(PLGA) to prepare the nanocomposites of PLLA-g-BG/PLGA with the various blend ratios of two phases. PLLA-g-BG accounted 10%, 20% and 40% in the composite, respectively. TGA, ESEM and EDX were used to analyze the graft ratio of PLLA-g-BG, the dispersion of nano-particles and the surface elements of the composites respectively. The rabbit osteoblasts were seeded and cultured on the thin films of composites in vitro. The cell adhesion, spreading and growth of osteoblasts were analyzed with FITC staining, NIH Image J software and MTT assay. The change of cell cycle was monitored by flow cytometry(FCM). The results demonstrated that the surface modification of BG with PLLA could significantly improve the dispersing of the particles in the matrix of PLGA. The nanocomposite with 20%PLLA-g-BG exhibited superior surface properties, including roughness and plenty of silicon, calcium and phosper, to enhance the adhesion, spreading and proliferation of osteoblasts. Our results indicate that the application of PLLA-g-BG with a certain blend ratio can improve the bioactivity and biocompatibility of biodegradable polyester.

Key words: Bioglass nanoparticle, Surface grafting, Poly(Lactide-co-glycolide)(PLGA), Nanocomposite, Bioactivity

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