高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

以PEG为间隔基固定赖氨酸制备血液相容的聚氨酯材料

胡小洋1, 张燕霞1, 张弢2, 于谦1, 陈红1   

    1. 武汉理工大学材料科学与工程学院, 武汉 430070;
    2. 南京大学材料科学与工程学院, 南京 210093
  • 收稿日期:2008-08-04 修回日期:1900-01-01 出版日期:2009-03-10 发布日期:2009-03-10
  • 通讯作者: 陈红

Lysine Immobilization Through Poly(ethylene glycol) Spacer for Bloodcompatible Polyurethane Surface

HU Xiao-Yang1, ZHANG Yan-Xia1, ZHANG Tao2, YU Qian1, CHEN Hong1*   

    1. School of Materials Science and Engineering, Wuhan University of Technology, Wuhan 430070, China;
    2. Department of Materials Science and Engineering, Nanjing University, Nanjing 210093, China
  • Received:2008-08-04 Revised:1900-01-01 Online:2009-03-10 Published:2009-03-10
  • Contact: CHEN Hong

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被引次数

15

摘要： 通过多步表面改性方法制备了血液相容性好的聚氨酯材料. 以PEG为间隔基将ε-赖氨酸通过Schiff碱反应和进一步的还原反应连接于聚氨酯表面. 该表面的水接触角和XPS结果表明, PEG和ε-赖氨酸成功接枝. 用蛋白质吸附和血栓溶解实验对材料的血液相容性进行了研究. 蛋白质吸附结果表明, 相对于改性前的聚氨酯, ε-赖氨酸改性后的表面能减少纤维蛋白原的吸附量近80%. 血栓溶解测试结果显示, ε-赖氨酸改性后的表面能够在13 min内使初生的血栓溶解. 这些结果证实, 改性后的表面不仅能抑制非特异性蛋白质的吸附, 而且在测试条件下能溶解初生的血栓.

关键词: 聚氨酯, 聚乙二醇, 赖氨酸, 抗蛋白吸附, 表面改性, 溶解血栓, 血液相溶性

Abstract: A blood-compatible polyurethane surface was prepared by multiply steps surface modification. ε-Lysine was conjugated to polyurethane surface through PEG spacer via Schiff-base and reduce reaction. The results of water contact angle and XPS measurements suggest that both PEG and lysine are immobilized to the surface successfully. Protein adsorption and plasma clot lysis measurements were performed to investigate the bloodcompatibility of the modified surface. Comparing with the unmodified surface, ε-lysine modified surface reduced fibrinogen adsorption by 80%. It was shown in clot lysis experiment that the ε-lysine modified surface could break down the incipient fibrinous clot within 13 min. The results demonstrate that ε-lysine modified surface not only suppress non-specific protein adsorption, but also degrade fibrin in an evolving clot.

Key words: Polyurethane, Poly(ethylene glycol), Lysine, Protein adsorption resistance, Surface modification, Clot lysis, Bloodcompatibility

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