高等学校化学学报

高等学校化学学报

• 研究快报 • 上一篇    下一篇

超亲疏水图案改善阵列芯片质量

阴启明, 叶嘉明, 周勇亮   

  1. 厦门大学化学化工学院化学系, 化学生物学福建省重点实验室, 厦门 361005
  • 收稿日期:2008-04-08 修回日期:1900-01-01 出版日期:2008-08-10 发布日期:2008-08-10
  • 通讯作者: 周勇亮

Improving Quality of Microarray Chip Based on Superhydrophilic-Superhydrophobic Patterned Surface

YIN Qi-Ming, YE Jia-Ming, ZHOU Yong-Liang*   

  1. Department of Chemistry, the Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
  • Received:2008-04-08 Revised:1900-01-01 Online:2008-08-10 Published:2008-08-10
  • Contact: ZHOU Yong-Liang

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被引次数

14

摘要： 本文提出在超疏水表面加工超亲水圆点图案为阵列基底制作免疫蛋白微阵列, 从而减轻“咖啡环效应”, 改善阵列芯片质量.

关键词: 阵列芯片, 咖啡环效应, 超亲水, 超疏水, 图案化

Abstract: Microarray chip including genechip has been of great application in fields of biology, quarantine, pharmacy and so on. Most of microarray chips were prepared by spotting aqueous droplet on the surface of substrate. Due to “coffee-ring stain effect”, there always was a denser perimeter where sample was concentrated during the process of droplet drying after spotting. So the ununiformity of spot caused by “coffee-ring stain effect” was one troublesome problem of the chip quality. Herein, superhydrophilic spot arrays on superhydrophobic AAO surface were fabricated to ease the “coffee-ring stain effect”. Furthermore, a protein array is prepared on this patterned surface where immunoreactions are carried out. As a result, spot uniformity is greatly improved in a wide range of concentration and volume.

Key words: Microarray chip, Coffee-ring stain effect, Superhydrophilicity, Superhydrophobicity, Patterning

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