高等学校化学学报

高等学校化学学报

• 研究快报 • 上一篇    下一篇

利用泰勒分析和动态涂层毛细管电泳系统快速测定蛋白质的扩散系数

左敏1,2, 陈义1   

    1. 中国科学院化学研究所生命分析化学实验室, 北京 100080;
    2. 中国科学院研究生院, 北京 100039
  • 收稿日期:2007-05-21 修回日期:1900-01-01 出版日期:2007-10-10 发布日期:2007-10-10
  • 通讯作者: 陈义

Fast Determination of Protein Diffusion Coefficient by Taylor Dispersion Analysis and Capillary Electrophoresis System

ZUO Min1,2, CHEN Yi1*   

    1. Laboratory of Analytical Chemistry for Life Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China;
    2. Graduate School, Chinese Academy of Sciences, Beijing 100039, China
  • Received:2007-05-21 Revised:1900-01-01 Online:2007-10-10 Published:2007-10-10
  • Contact: CHEN Yi

PDF (PC)

被引次数

7

摘要： 以泰勒分散理论为基础, 首次采用动态涂层毛细管来准确和快速测定蛋白质分子的扩散系数.

关键词: 微量蛋白质, 扩散系数, 毛细管电泳, 泰勒分析

Abstract: A method for the fast and accurate measurement of diffusion coefficient of minute proteins was developed. A protein sample plug was introduced into the capillary and forced to flow through a capillary coated dynamically by poly(diallyldimethylammonium chloride). Its concentration profile was then monitored by the UV absorption detector mounted on Beckman MDQ capillary electrophoresis system. Accurate diffusion coefficient was obtained. The key conditions for the accurate measurement include: (1) systematic peak should be developed in a laminar flow; (2) radius diffusion is negligible compared with the longitudinal diffusion; (3) the capillary is well thermostated and (4) the solute should not be overloaded. In common case, the deviation of the measurement was kept within 4%.

Key words: Minute protein, Diffusion coefficient, Capillary electrophoresis, Taylor analysis

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