高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

电喷雾解吸电离质谱法用于临床尿样的直接分析

陈焕文1, 李明1, 周建光1, 费强1, 姜杰1, 金钦汉1, 张天幕2, 张燮3   

    1. 吉林大学化学学院, 吉林省光谱仪器分析仪器工程技术研究中心, 长春 130021;
    2. 吉林大学中日联谊医院, 长春 130039;
    3. 东华理工学院应用化学系, 抚州 344000
  • 收稿日期:2005-08-22 修回日期:1900-01-01 出版日期:2006-08-10 发布日期:2006-08-10
  • 通讯作者: 金钦汉

Desorption Electrospray Ionization Mass Spectrometry for Direct Analysis of Clinic Urine Samples

CHEN Huan-Wen1, LI Ming1, ZHOU Jian-Guang1, FEI Qiang1, JIANG Jie1, JIN Qin-Han1, ZHANG Tian-Mu2, ZHANG Xie3   

    1. College of Chemistry, Jilin Province Research Center of Engineering and Technology for Spectral Instruments, Jilin University, Changchun 130021, China;
    2. China-Japan Union Hospital, Jilin University, Changchun 130039, China;3. Applied Chemistry Department, East China Institute of Technology, Fuzhou 344000, China
  • Received:2005-08-22 Revised:1900-01-01 Online:2006-08-10 Published:2006-08-10
  • Contact: JIN Qin-Han

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被引次数

51

摘要： 将电喷雾解吸电离质谱(DESI-MS)用于临床尿样的分析, 优化了电喷雾溶剂流速、电喷雾电压和喷雾锥距离等重要参数. 采用普通滤纸作为样品载体, 在不需要样品预处理的前提下同时快速测定了临床尿样中的钾、钠、尿素、尿酸、丙酮酸和肌苷等多种成分, 并对各种成分的主要离子进行了串联质谱鉴定. DESI-MS在进行多组分同时测定时不需要进行样品预处理, 缩短了测定时间, 单个样品的分析时间不到1 min. 同时, 采用内标法对所测定组分进行了半定量分析.

关键词: 电喷雾电离质谱, 尿样, 串联质谱, 同时测定

Abstract: DESI-MS was applied to direct simultaneous detection of multiple components of clinic urine samples without any sample pretreatment. Under the optimized working conditions, such as ESI infusion rate, ESI high voltage, incident angle, collective angle, tube lens voltage, etc., for both DESI and LTQ mass spectro-meter, the main components of urine including urea, pyruvic acid, creatinine, oxalic acid, alloxane, menadione were detected as the protonated molecules in positive detection mode using methanol-water(volume ratio 1:1) as the spray solvent in the low mass range(15—200 amu). Sodium and potassium were also detected but mainly in the form of clusters with urea and creatinine. In each case, the corresponding ions were isolated in the ion trap mass analyzer and underwent a collision-induced dissociation process, which yielded characteristic fragments in tandem mass spectra and resulted in confident validation of the analytical results. The average time for single sample analysis was less than 1 min since it required no sample pretreatment. Furthermore, the semi-quantitative analysis of the components was also demonstrated by adding serine as an internal standard into the samples.

Key words: DESI-MS, Clinic urine sample, Tandem mass spectrometry, Simultaneous detection

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