高等学校化学学报

高等学校化学学报

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红细胞标记抗体的化学发光免疫分析

张雯艳1,2, 魏景艳1, 郭杰3, 陈桂秋4, 邢程3, 石立华5, 袁玮2, 钱莉娜2, 阙肖冬2, 高莉2, 刘梦虹2   

  1. 1. 吉林大学药学院,长春 130021; 2. 吉林省临床检验中心, 长春 130021; 3. 吉林大学公共卫生学院, 长春 130021; 4. 吉林大学中日联谊医院, 长春 130031; 5. 临江市医院, 临江 134600
  • 收稿日期:2005-10-28 修回日期:1900-01-01 出版日期:2006-08-10 发布日期:2006-08-10
  • 通讯作者: 魏景艳

Studies on a Chemiluminescent Immunoassay with the Erythrocyte-labeled Antibody

ZHANG Wen-Yan1,2, WEI Jing-Yan1, GUO Jie3, CHEN Gui-Qiu4, XING Cheng3, SHI Li-Hua5, YUAN Wei2, QIAN Li-Na2, QUE Xiao-Dong2, GAO Li2, LIU Meng-Hong2   

    1. College of Pharmaceutical Science, Jilin University, Changchun 130021, China;
    2. Jilin Province Center of Clinical Laboratory, Changchun 130021, China;
    3. College of Public Health, Jilin University, Changchun 130021, China;
    4. China-Japan Union Hospital, Jilin University, Changchun 130031, China;
    5. Linjiang Hospital, Linjiang 134600, China
  • Received:2005-10-28 Revised:1900-01-01 Online:2006-08-10 Published:2006-08-10
  • Contact: WEI Jing-Yan

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被引次数

10

摘要： 用红细胞代替辣根过氧化物酶作为双抗体夹心免疫分析中第二抗体的标记物, 建立了一种红细胞标记抗体的免疫化学发光测定乙型肝炎病毒表面抗原的新方法. 在免疫反应完成后, 结合了抗原-抗体免疫复合物的致敏红细胞在低渗溶液中溶血, 释放出血红蛋白. 基于血红蛋白对鲁米诺-H2O2体系化学发光具有催化作用的原理, 采用化学发光法测定血红蛋白含量. 测得的血红蛋白发光强度与待测抗原浓度呈线性关系. 采用这种方法可检测出0.5 ng/mL的乙型肝炎病毒表面抗原. 将该方法与酶联免疫吸附分析(ELISA)结合起来对乙型肝炎患者血清乙肝病毒表面抗原(HBsAg)进行检测, 两者符合率均为97%, 表明本法具有良好的灵敏度和特异性, 可用于临床标本测试.

关键词: 红细胞标记抗体, 化学发光免疫分析, 乙型肝炎病毒表面抗原.

Abstract: A novel chemiluminescence immunoassay suitable for the determination of the hepatitis B virus' surface antigen(HBsAg) was studied. Instead of the enzyme-labeled antibody in enzyme-lined immunosorbent assay(ELISA), the erythrocyte-labeled monoclonal anti-HBsAg antibodies were utilized as the second antibody in this double antibody sandwich immunoassay. After the sandwich immunoreaction occurs, the sensitized erythrocyte, having combined with the immune complex made from antigen and the first antibody, suffers from hemolysis in low osmotic solution and releases hemoglobin(Hb). Hb can catalyze the chemiluminescent reaction of luminal by hydrogen peroxide. The intensity of the chemiluminescent reaction was linearly with the concentration of the hepatitis B virus' surface antigen(HBsAg) in the detected samples. This assay can be used to detect 0.5 ng/mL of HBsAg. The 30 healthy donors and 30 patients with hepatitis B were detected for HBsAg with this method and with routine ELISA as the standard. The coincidence rate of the two assays was 97%, which illustrates that the developed chemiluminescence immunoassay with erythrocyte-labeled antibody is suitable in clinical determination.

Key words: Erythrocyte-labeled antibody, Chemiluminescent immunoassay, Hepatitis B virus’ surface antigen

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