高等学校化学学报

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毛细管电泳高频电导法测定几种中药中的丁香酚

林伟丰1, 吴小林2, 陈缵光3, 李全文3, 蔡沛
祥1, 莫金垣1
  

    1. 中山大学化学院, 广州 510275;
    2. 中山大学附属笫三医院, 广州 510630;
    3. 中山大学药学院, 广州 510089
  • 收稿日期:2006-04-13 修回日期:1900-01-01 出版日期:2006-11-10 发布日期:2006-11-10
  • 通讯作者: 陈缵光

Determination of Eugenol in Several Chinese Herbal Medicines by Capillary Electrophoresis with High Frequency Conductivity Method

LIN Wei-Feng1, WU Xiao-Lin2, CHEN Zuan-Guang3, LI Quan-Wen3, CAI Pei-Xiang1, MO Jin-Yuan1   

    1. School of Chemistry, Sun Yat-Sen University, Guangzhou 510275, China;
    2. The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510630, China;
    3. School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510089, China
  • Received:2006-04-13 Revised:1900-01-01 Online:2006-11-10 Published:2006-11-10

摘要: 在三乙胺的弱碱性体系中, 对几种不同产地中药中的丁香酚含量进行了测定, 结果显示这几种中药中的丁香酚含量存在明显的地区差异.

关键词: 毛细管电泳, 高频电导检测, 非接触式电导检测, 丁香酚, 中草药

Abstract: A method based on capillary electrophoresis with a high frequency conductivity method was developed for the determination of eugenol in several Chinese herbal medicines from different producing areas. In this study, some key factors for separation and detection were studied and the best analysis conditions were obtained. In the buffer system of 2.0 mmol/L triethylamine +0.74 mol/L methanol at separation potential of 18.0 kV, the optimal separation and detection wasobtained. Under the chosen conditions, eugenol was eluted within 7 min. The linear range of eugenol was 2.0—540 μg/mL, the limit of detection was 0.6 μg/mL, and the recovery ranged from 93.3% to 106%.

Key words: Capillary electrophoresis, High frequency conductivity detection, Cont actless conductivity detection, Eugenol, Chinese herbal medicine

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