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芍药苷的电喷雾串联质谱研究

董红娟1,2, 刘志强1, 宋凤瑞1, 于湛1, 李惠琳1, 刘淑莹1
  

    1. 中国科学院长春应用化学研究所, 长春质谱中心, 长春 130022;
    2. 中国科学院研究生院, 北京 100049
  • 收稿日期:2005-12-05 修回日期:1900-01-01 出版日期:2006-11-10 发布日期:2006-11-10
  • 通讯作者: 刘志强

Studies on Paeoniflorin by Electrospray Ionization Tandem Mass Spectrometry

DONG Hong-Juan1,2, LIU Zhi-Qiang1, SONG Feng-Rui1, YU Zhan1, LI Hui-Lin1, LIU Shu-Ying1   

    1. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun Center of Mass Spectrometry, Changchun 130022, China;
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100049, China
  • Received:2005-12-05 Revised:1900-01-01 Online:2006-11-10 Published:2006-11-10
  • Contact: LIU Zhi-Qiang

摘要: 采用电喷雾串联质谱(ESI-MSn)技术, 结合H/D交换方法, 在正、负离子检测模式下对白芍药材中主要成分芍药苷的质谱裂解规律进行了系统研究. 实验结果表明, 该化合物在正、负离子模式下均得到较好的质谱信息, 且在正离子模式下, 电喷雾质谱分析的灵敏度更高. 同时获得了其质谱裂解规律, 为白芍中其它化合物的分析鉴定提供了有效的质谱方法.

关键词: 芍药苷, 电喷雾串联质谱(ESI-MSn), H/D交换, 裂解机理

Abstract: Paeoniflorin was studied by using Electrospray Ionization Tandem Mass Spectrometry(ESI-MSn) both in positive and negative modes. Through the experimental method of hydrogen and deuterium exchange, the collision-induced dissociation fragmentation pathways were described and it proved that the precursor ion of paeoniflorin was clines to loss one molecule benzoic acid in both positive and negative MS2 spectra, furthermore, the loss of glucose residue was observed in positive ion mode, while, only one formaldehyde molecule loss from C-6 of glucose was observed in negative ion mode. The established method could be used for the sensitive and rapid identification of monoterpene glycoside in Paeonia albiflora.

Key words: Paeoniflorin, Electrospray Ionization Tandem Mass Spectrom etry(ESI-MSn), H/D exchange, Fragmentation mechanism

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