高等学校化学学报 ›› 2006, Vol. 27 ›› Issue (1): 71-74.

• 研究论文 • 上一篇    下一篇

一种海洋弧菌(Vibrio sp.  WYA)褐藻胶裂合酶降解特性研究

张真庆, 于广利, 赵峡, 王远红, 吕志华, 管华诗   

  1. 中国海洋大学海洋药物与食品研究所, 青岛 266003
  • 收稿日期:2004-12-06 出版日期:2006-01-10 发布日期:2006-01-10
  • 通讯作者: 于广利(1964年出生), 男, 教授, 博士生导师, 主要从事糖化学与糖生物学研究. E-mail: glyu@ouc.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 30370345)、 国家重点基础研究发展计划(批准号: 2003CB716401)以及青岛科技局项目(04-2-JS-133)资助.

Degradation Character of a Alginate Lyase from Vibrio sp.  WYA

 ZHANG Zhen-Qing, YU Guang-Li, ZHAO Xia, WANG Yuan-Hong,LV Zhi-Hua, GUAN Shi-Hua   

  1. Marine Drug and Food Institute, Ocean University of China, Qingdao 266003, China
  • Received:2004-12-06 Online:2006-01-10 Published:2006-01-10
  • Contact: YU Guang-Li,E-mail: glyu@ouc.edu.cn

摘要:

从海洋弧菌(Vibrio sp.  WYA)中得到一种褐藻胶裂合酶, 将其分别作用于寡聚甘露糖醛酸和古罗糖醛酸纯品(dp5~7)以及褐藻胶, 采用HPTLC和FPLC等技术对产物进行分析, 并应用ESI\|MS和NMR进行结构分析. 结果表明, 该酶最小识别片段为六糖, 终产物主要为三糖, 且识别和切割位点为甘露糖醛酸残基.

关键词: 褐藻胶; 寡糖; 褐藻胶裂合酶; 海洋弧菌;  三糖酶解机理

Abstract:

The characterization of an alginate lyase, which was purified from Vibrio sp.  WYA, was studied.  This enzyme can specific ally degrade alginate to produce oligosaccharides, the oligosaccharides was separated by BioGel P6 column, and purified by Mono Q column.  The degree of polymerization and structure of these oligosaccharides were confirmed by HPTLC, ESIMS and NMR methods, respectively.  The results show that, all the oligosaccharides are trimers, and have structures of DGG, DMG and DMM, respectively. The minimal recognition and hydrolysis fragment of this enzyme is hexasaccharide, and the degradation site occurred on the non\|reducing end mannuronic acid of the polysaccharide.  The degradation mechanism of this kind of enzyme is very unique, and very useful for the alginate structure analysis, and alginate trisaccharides preparation. 

Key words: Alginate; Oligasaccharide; Alginate lyase; Vibrio sp. WYA; Trisaccharide degradation mechanism

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