高等学校化学学报 ›› 2006, Vol. 27 ›› Issue (1): 67-70.

• 研究论文 • 上一篇    下一篇

大孔吸附树脂法去除淫羊藿多糖中蛋白的研究

李瑞军, 李德耀, 张现峰, 任冬春, 刘宏民   

  1. 郑州大学化学系, 郑州大学新药研究开发中心, 郑州 450052
  • 收稿日期:2004-12-21 出版日期:2006-01-10 发布日期:2006-01-10
  • 通讯作者: 刘宏民(1960年出生), 男, 博士, 教授, 博士生导师, 主要从事天然药化、 药物化学和糖化学方面的研究. E-mail: liuhm@zzu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:  20272054)资助.

Deproteinization from Epimedium Polysaccharide by Macroporous Adsorption Resin

 LI Rui-Jun, LI De-Yao, ZHANG Xian-Feng, LIN Dong-Chun, LIU Hong-Min   

  1. Department of Chemistry, New Drug Research & Development Center of Zhengzhou University, Zhengzhou 450052, China
  • Received:2004-12-21 Online:2006-01-10 Published:2006-01-10
  • Contact: LIU Hong-Min,E-mail: liuhm@zzu.edu.cn

摘要:

从4种大孔吸附树脂中筛选出ADS-7, 考察了其对淫羊藿多糖中蛋白的去除作用, 并讨论了pH值、 鞣酸、 上样量等对树脂去蛋白效率的影响. 结果表明, 该方法对淫羊藿粗多糖中的蛋白具有较高的去除效率, 淫羊藿粗多糖中的蛋白含量由1.2%下降到0.035%.

关键词: 大孔吸附树脂; 淫羊藿多糖; 脱蛋白

Abstract:

To deproteinize from crude Epimedium polysaccharide, ADS-7 type of resin was selected from four kinds of macroporous adsorption resins.  The influence factors, such as pH value, tannic Acid, elution volume and so on, were investigated.  The content of proteins in crude Epimedium polysaccharide decreased from 1.2% to 0.035%.  The results show that deproteinization from crude Epimedium polysaccharide by macroporous adsorption resin is a feasible method.

Key words: Macroporous adsorption resin; Epimedium polysaccharide; Deproteinization

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