高等学校化学学报

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (9): 1612.

• 研究论文 • 上一篇    下一篇

单细胞中D-天冬氨酸和D-谷氨酸的毛细管电泳分析

赵书林, 沈江珊   

  1. 广西师范大学化学化工学院, 桂林541004
  • 收稿日期:2004-09-08 出版日期:2005-09-10 发布日期:2005-09-10
  • 基金资助:

    国家自然科学基金(批准号:20265001);广西省自然科学基金(批准号:0236013)资助.

Analysis of D-Aspartic Acid and D-Glutamic Acid in Single Cells by Capillary Electrophoresis

ZHAO Shu-Lin, SHEN Jiang-Shan   

  1. College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 541004, China
  • Received:2004-09-08 Online:2005-09-10 Published:2005-09-10

PDF (PC)

被引次数

16

摘要： 建立了一种毛细管电泳-激光诱导荧光检测单个神经细胞中D-天冬氨酸和D-谷氨酸的方法.以萘二甲醛(NDA)为衍生试剂,β-环糊精(β-CD)和脱氧胆酸钠(SDC)为拆分体系,对16对氨基酸对映体的NDA衍生物进行了手性拆分研究.考察了蛋白氨基酸和一些胺对检测D-天冬氨酸和D-谷氨酸的影响,并确立了最佳分离条件,从而建立了单细胞中D-天冬氨酸和D-谷氨酸的检测方法,并对海兔的单个神经细胞中D-天冬氨酸和D-谷氨酸进行了检测.

关键词: D-天冬氨酸, D-谷氨酸, 手性分离, 单细胞分析, 毛细管电泳

Abstract: An analytical method was established to detect D-aspartic acid(D-Asp) and D-glutamic acid(D-Glu) in single cells. Cellular samples were derivatized with a fluorescence tagging reagent, naphthalene-2,2-dicarboxaldehyde in a mini-volume(3 μL) prior to the separation. Sixteen pair of amino acid enantiomers resolution was achieved by using a dual chiral selector system consisting of β-cyclodextrin(β-CD) and chiral micelles formed by sodium deoxycholate(SDC). The separation was coupled with laser induced fluorescence detection. Single neurons isolated from the ganglia of Aplysia were analyzed to determine the D/(D+L) molar ratios of Asp to Glu. D-Asp and D-Glu were found at high levels and its cellular distribution was highly heterogeneous.

Key words: D-Aspartic acid, D-Glutamic acid, Chiral separation, Single cells analysis, Capillary electrophoresis

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