高等学校化学学报

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (7): 1242.

• 研究论文 • 上一篇    下一篇

牛蒡寡糖的分离纯化及结构研究

郝林华1, 陈磊2, 仲娜3, 陈靠山1,4, 李光友1   

  1. 1. 国家海洋局一所海洋生物活性物质重点实验室,青岛266061;
    2. 青岛市立医院,青岛266011;
    3. 青岛市药品检验所,青岛266071;
    4. 山东大学生命科学学院,济南250100
  • 收稿日期:2004-08-04 出版日期:2005-07-10 发布日期:2005-07-10
  • 通讯作者: 陈靠山(1962年出生),男,博士,教授,博士生导师,从事植物生理与病理、生物防治植物病害的研究.E-mail:ksc313@263.net E-mail:ksc313@263.net
  • 基金资助:

    国家“八六三”计划(批准号:2002AA244031);国家海洋局基金(批准号:03-008);青岛市科技局计划(批准号:02-2-kj-zhj-81)资助

Separation, Purification and Structure of Burdock Oligosaccharide

HAO Lin-Hua1, CHEN Lei2, ZHONG Na3, CHEN Kao-Shan1,4, LI Guang-You1   

  1. 1. Key Lab of Marine Bioactivity Substance of State Oceanic Administration, Qingdao 266061, China;
    2. Municipal Hospital of Qingdao City, Qingdao 266011, China;
    3. Qingdao Institute for Drug Control, Qingdao 266071, China;
    4. Life Science Institute, Shandong University, Jinan 250100, China
  • Received:2004-08-04 Online:2005-07-10 Published:2005-07-10

PDF (PC)

被引次数

148

摘要： 以菊科植物牛蒡(ArctiumlappaL.)的等外根为材料,经热水浸提、木瓜蛋白酶-Sevag法脱蛋白、乙醇沉淀和SephadexG-50凝胶柱层析分离纯化,得到水溶性的牛蒡寡糖(BOS2).采用化学分析方法研究了BOS2的理化性质,并通过TLC,GC-MS,HP-GPC,UV,IR,1HNMR及13CNMR等对BOS2的纯度、结构和组成进行表征.结果表明,BOS2由果糖和葡萄糖组成,摩尔比约为12∶1,纯度较高,为均一性组分;由12个呋喃型的果糖以β(2→1)糖苷键相连,1个吡喃型的葡萄糖以α(1→2)糖苷键连接在果糖末端的线性直链结构,是一种菊糖构型的低聚果糖.

关键词: 牛蒡寡糖, 分离纯化, 结构分析

Abstract: A kind of soluble oligosaccharide, Burdock oligosaccharide(BOS2), was extracted by hot water, deproteinized by enzymolysis and Sevag method, precipated by ethanol, separated and purified by Sephadex G-50 column chromatography from burdock sub-standard root. Its purification, physical and chemical properties and structure were studied by means of some chemical reactions assay and instrumental analysis. The results show that BOS2 is composed of D-fructose and D-glucose in a molar ratio of about 12∶1, which is confirmed by the composition of 12 fructose residues linked by β(2→1) glucoside bond and 1 glucose residue linked by α(1→2) glucoside bond at the end of linear straight sugar chain. It is shown to be a single homogeneous component.

Key words: Burdock oligosaccharide, Separation and Purification, Structure analysis

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