高等学校化学学报

高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (02): 308.doi: 10.3969/j.issn.0251-0790.2012.02.017

• 生物化学 • 上一篇    下一篇

海地瓜蛋白水解物中ACE抑制肽的分离纯化及合成

赵元晖1, 李八方1, 马敬军2, 董士远1, 刘尊英1, 曾名湧1   

  1. 1. 中国海洋大学食品科学与工程学院, 青岛 266003;
    2. 中国检验认证集团山东有限公司, 青岛 266071
  • 收稿日期:2011-02-23 出版日期:2012-02-10 发布日期:2012-01-13
  • 通讯作者: 曾名湧, 男, 博士, 教授, 主要从事水产品高值化利用研究. E-mail: mingyz@mail.ouc.edu.cn 赵元晖, 男, 博士, 讲师, 主要从事海洋生物活性物质研究. E-mail: zhaoyuanhui@ouc.edu.cn E-mail:mingyz@mail.ouc.edu.cn;zhaoyuanhui@ouc.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 31000830)和山东省自然科学基金(批准号: ZR2010CQ001)资助.

Purification and Synthesis of ACE Inhibitory Peptide from Acaudina molpadioidea Protein Hydrolysate

ZHAO Yuan-Hui1, LI Ba-Fang1, MA Jing-Jun2, DONG Shi-Yuan1, LIU Zun-Ying1, ZENG Ming-Yong1   

  1. 1. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China;
    2. China Certification and Inspection Group Shandong Co. Ltd., Qingdao 266071, China
  • Received:2011-02-23 Online:2012-02-10 Published:2012-01-13
  • Contact: zhao yuanhui E-mail:mingyz@mail.ouc.edu.cn;zhaoyuanhui@ouc.edu.cn

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被引次数

41

摘要： 采用Sephadex G-25凝胶柱层析、SP Sephadex C-25 阳离子交换层析和反相高效液相色谱等方法对海地瓜水解产物进行分离纯化, 得到了一种新的强活性ACE抑制肽, 其氨基酸序列为MEGAQEAQGD, IC50值为15.9 μmol/L. 采用逐步缩合和片段缩合的方法对该抑制肽进行了设计合成. 合成肽的纯度为99.72%, 分子量与序列结构均与理论值相符. 研究发现, 抑制肽与胃蛋白酶和糜蛋白酶水解反应后, 活性增强了3.5倍. 动物实验结果表明, 剂量为3 μmol/kg的抑制肽对大鼠自发性高血压具有明显的降压效果.

关键词: 海地瓜, ACE抑制肽, 分离纯化

Abstract: A novel ACE inhibitory peptide was isolated from the sea cucumber(Acaudina molpadioidea) hydrolysate using the chromatographic methods including gel filtration, ion-exchange chromatography and reversed phase high-performance liquid chromatography. The purified ACE inhibitory peptide was sequenced as MEGAQEAQGD, with a IC50 value of 15.9 μmol/L. The ACE inhibitory peptide was synthesized by a method of gradual condensation and fragmental condensation. The purity of the decapeptide was 99.72%, and the molecular weight and sequence structure of the decapeptide equate with the fact. It was found that the inhibitory activity of the peptide was intensified by 3.5 times after incubation with pepsin and chymotrypsin. The ACE inhibitory peptide from Acaudina molpadioidea showed a clear antihypertensive effect on spontaneously hypertensive rats(SHR), at a dosage of 3 μmol/kg.

Key words: Acaudina molpadioidea, ACE inhibitory peptide, Isolation and purification

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