高等学校化学学报

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (6): 997.

• 研究论文 • 上一篇    下一篇

水溶性锌卟啉配合物的合成、表征及其与CTDNA的作用

康敬万, 吴海霞, 卢小泉, 苏碧泉, 卓琳   

  1. 西北师范大学化学化工学院, 兰州730070
  • 收稿日期:2004-08-16 出版日期:2005-06-10 发布日期:2005-06-10
  • 通讯作者: 康敬万(1938年出生),男,教授,博士生导师,主要从事电分析化学研究.E-mail:jwkang@nwnu.edu.cn E-mail:jwkang@nwnu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:20275031,20335030);西北师范大学创新工程(批准号:KJCXGC-01)资助.

Synthesis and Characterization of Water-soluble Zinc Porphyrin Complex and Its Interaction with CT DNA

KANG Jing-Wan, WU Hai-Xia, LU Xiao-Quan, SU Bi-Quan, ZHOU Lin   

  1. Department of Chemistry, Northwest Normal University, Lanzhou 730070, China
  • Received:2004-08-16 Online:2005-06-10 Published:2005-06-10

PDF (PC)

被引次数

37

摘要： 合成了5,10,15,20-四[4-(3'-丙氧基吡啶溴化盐)苯基]卟啉(TPPBr)及其锌卟啉配合物TPPBr(Zn),用红外光谱、核磁共振和紫外光谱进行了表征.利用紫外-可见光谱、荧光光谱、圆二色谱和黏度实验研究了TPPBr(Zn)与小牛胸腺DNA(CTDNA)的相互作用,并计算了TPPBr(Zn)与CTDNA作用的表观键合常数为2.64×105L/mol.进而证明了TPPBr(Zn)与CTDNA以外部自堆积和静电结合的混合模式作用.

关键词: 水溶性锌卟啉, DNA, 自堆积和静电结合模式

Abstract: A porphyrin 5,10,15,20-tetrakisporphyrin and its Zn complex(TPPBr(Zn)) were synthesized and characterized. The interaction of TPPBr(Zn) with CT DNA was studied by UV-Vis spectroscopy, fluorescence spectra, circular dichroic spectrum and viscosity measurements. The constant of TPPBr(Zn) binding to CT DNA is 2.64×105 L/mol. The results suggest that the porphyrin complex can bind to DNA by self-stacked outside binding and static binding.

Key words: Water-soluble zinc porphyrin, DNA, Self-stack and static-binding

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