高等学校化学学报

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (6): 991.

• 研究论文 •    下一篇

核磁共振技术结合模式识别对中药马兜铃酸亚急性生化效应的研究

张晓宇, 吴惠丰, 裴奉奎, 倪嘉缵   

  1. 稀土化学和物理重点实验室, 中国科学院长春应用化学研究所, 中国科学院研究生院, 长春130022
  • 收稿日期:2004-11-15 出版日期:2005-06-10 发布日期:2005-06-10
  • 通讯作者: 倪嘉缵(1931年出生),男,研究员,博士生导师,中国科学院院士,从事稀土及化学生物学研究.E-mail:zxy@ciac.jl.cn E-mail:zxy@ciac.jl.cn

Studies on Subacute Biochemical Effects of Aristolochic Acid Using 1H NMR Spectroscopy Combined with Pattern Recognition

ZHANG Xiao-Yu, WU Hui-Feng, PEI Feng-Kui, NI Jia-Zuan   

  1. Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Graduate School of Chinese Academy of Sciences, Changchun 130022, China
  • Received:2004-11-15 Online:2005-06-10 Published:2005-06-10

PDF (PC)

被引次数

37

摘要： 利用核磁共振技术结合模式识别方法,研究了马兜铃酸(AristolochicAcid,AA)的亚急性生化效应.大鼠连续5日腹腔注射马兜铃酸后,不同时间段尿液1HNMR谱显示与肾小管及肾乳头受损相关的标记物(NMRmarker)浓度有显著变化;铬酸钠、氯化汞、二溴乙胺、盐酸肼和异硫氰酸-α-萘酯,并利用主成分分析法对造成的肝肾损伤模型组、AA组和对照组的大鼠尿液1HNMR谱解析和分类.1HNMR谱中各种代谢物的谱峰强度变化及主成分分析结果均显示,马兜铃酸引起的肾损伤与肾小管及肾乳头损伤模型类似,且随给药量的积累,肾损伤范围扩大程度加深,引起肾脏不可逆损伤.该方法可用于中药的毒理学研究.

关键词: 马兜铃酸, 体液, 核磁共振, 模式识别, 毒性

Abstract: 1HNMR spectroscopy and pattern recognition method were used to the studies on the subacute biochemical effects of aristolochic acid(AA). Male Wistar rats were intraperitoneal with AA for 5 days. Urine samples were collected after being treated with AA and the NMR spectra show the NMR marker related to renal papillary and proximal tubular lesion changed greatly. Urine samples from the rats treated with model toxins sodium chromate, mercury Ⅱ chloride, 2-bromoethanamine hydrobromide, α-naphthylisothiocyanate and hydrazine(NaCrO4, HgCl2, BEA, ANIT and HYD) were collected over a 48 h time course. Each NMR spectrum was data-processed and analyzed by PCA methods. A successful classification of the toxicity and biochemical effects of AA was achieved. The biochemical effects and PCA classification indicate that the renal toxicity of AA was a progressive course with the accumulation of AA, and caused widespread lesion to kidney including proximal tubule and papillary necrosis. The lesion is irreversible. This method can be used in the toxicological study of other drugs.

Key words: Aristolochic acid, Biofluids, NMR, Pattern recognition, Toxicity

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