高等学校化学学报

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (6): 1166.

• 研究论文 • 上一篇    下一篇

石英晶体微天平实时监测低密度脂蛋白在胆固醇修饰葡聚糖上的吸附及其动力学研究

韩松岩1, 刘东波1, 黄亮1, 王深琪2, 刘清平2, 安齐顺一3, 长哲郎3, 陈强1   

  1. 1. 南开大学生命科学学院;
    2. 南开大学生物活性材料教育部重点实验室, 天津300071;
    3. 日本东北大学大学院药学研究科, 仙台9808578
  • 收稿日期:2004-06-15 出版日期:2005-06-10 发布日期:2005-06-10
  • 通讯作者: 陈　强(1962年出生),男,博士,教授,博士生导师,主要从事生物传感器、中药药理及药物分析及生物活性材料等领域研究.E-mail:qiangchen@nankai.edu.cn E-mail:qiangchen@nankai.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:90209054);国家“八六三”项目(批准号:2002AA326060);南开大学、天津大学科技合作基金项目资助.

Monitoring Adsorption in situ and Kinetic Studies of Low-density Lipoprotein onto Cholesterol Modified Dextran Using Quartz Crystal Microbalance

HAN Song-Yan1, LIU Dong-Bo1, HUANG Liang1, WANG Shen-Qi2, LIU Qing-Ping2, Anzai Jun-ichi3, Tetsuo Osa3, CHEN Qiang1   

  1. 1. Life Sciences College;
    2. The Key Laboratory of Bioactive Materials of Ministry of Education, Nankai University, Tianjin 300071, China;
    3. Graduate School of Pharmaceutical Sciences, Tohoku University, Seidai 9808578, Japan
  • Received:2004-06-15 Online:2005-06-10 Published:2005-06-10

PDF (PC)

被引次数

4

摘要： 利用石英晶体微天平实时监测低密度脂蛋白(LDL)在胆固醇修饰葡聚糖(CMD)上的吸附,并对其吸附动力学进行研究.结果表明,CMD与LDL之间的相互作用符合Langmuir吸附方程,当LDL浓度(ng/μL)为9.9,12.38和14.14时,其吸附平衡常数[K/(mol·L1·s1)]分别为0.0477,0.0536和0.0628,表面吸附量(ng/cm2)分别为107.6,139.6和167.9.最大吸附量达到284.8ng/cm2,吸附率为72.91%.

关键词: 石英晶体微天平, 胆固醇修饰葡聚糖, 低密度脂蛋白, 吸附

Abstract: The use of quartz crystal microbalance(QCM) for monitoring in situ the adsorption of low-density lipoprotein(LDL) onto cholesterol modified dextran(CMD) was described. The kinetic studies were made. The experimental results show that kinetic curves are fitted for that of Langmuir adsorption equation quite well, when LDL mass concentration(ng/μL) are 9.9, 12.38, 14.14, respectively, and the rate constants for adsorption(mol·L-1·s-1) and adsorption capacity(ng/cm2) are estimated to be (0.047 7), 0.053 6, 0.062 8 and 107.6, 139.6, 167.9, respectively. The maximal LDL adsorption capacity attains 284.8 ng/cm2 and the adsorptive efficiency was calculated as 72.91%.

Key words: Quartz crystal microbalance, Cholesterol modified dextran, Low-density lipoprotein, (Adsorption)

中图分类号: 

TrendMD: