高等学校化学学报

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (5): 964.

• 研究论文 • 上一篇    下一篇

环氧化合物与丝素蛋白化学交联凝胶的结构

闵思佳1, 陈芳芳1, 吴豪翔2   

  1. 1. 浙江大学动物科学学院生物资源科学系;
    2. 浙江大学环境与资源学院, 杭州310029
  • 收稿日期:2004-05-13 出版日期:2005-05-10 发布日期:2005-05-10
  • 通讯作者: 闵思佳(1957年出生),女,博士,副教授,从事天然高分子材料研究.E-mail:minsj@zju.edu.cn E-mail:minsj@zju.edu.cn
  • 基金资助:

    浙江省自然科学基金(批准号:399474);杭州市“5112工程专项”(批准号:2003133E39)资助.

Structure of Chemical Cross-linked Gel of Epoxide with Silk Fibroin

MIN Si-Jia1, CHEN Fang-Fang1, WU Hao-Xiang2   

  1. 1. Department of Biological Resource Science, College of Animal Science;
    2. College of Environmental and Resource Science, Zhejiang University, Hangzhou 310029, China
  • Received:2004-05-13 Online:2005-05-10 Published:2005-05-10

PDF (PC)

被引次数

66

摘要： 对丝素蛋白水溶液与环氧化合物交联剂(PGDE)反应制备的丝素凝胶(CFG)的结构进行了研究.用位相差显微镜观察到CFG具有整体均一的形态结构,而未经PGDE交联的纯丝素凝胶(FG)为颗粒聚集结构.CFG的红外吸收光谱在1104cm-1处出现PGDE分子中烷醚的吸收峰,而没有出现PGDE两端环醚的特征吸收峰,显示PGDE已经开环交联在丝素蛋白上.氨基酸分析结果显示,酪氨酸、组氨酸和赖氨酸的含量与反应前相比明显减少,PGDE在这些位点与丝素蛋白发生了交联反应.固体13CNMR谱观察到酪氨酸羟苯基上的碳发生了化学位移,进一步证实了酪氨酸残基与PGDE发生了化学交联.研究表明,PGDE与丝素蛋白交联形成了分子间交联网络结构,从而使CFG成为较为匀质的凝胶,并有较好的透明度和柔韧性.

关键词: 丝素蛋白, 凝胶, 化学交联, 环氧化合物

Abstract: The structure of cross-linked fibroin gel(CFG) prepared by the reaction of cross-linking (agent) epoxide polyethylene glycol diglycidyl ether(PGDE) with silk fibroin solution was investigated. The results indicate that CFG express the whole homogeneous morphological structure observed by site microscopy phase contrast, while the pure fibroin gel(FG), which hadn't been cross-linked with PGDE, showed the particle-piled structure. There was an absorption peak of alkyl ether in PGDE appearing at 1 104 cm-1 of the FTIR spectrum of CFG, while it didn't show the characteristic peak of epoxide in both sides of PGDE. It revealed that PGDE has been cross-linked with silk fibroin by opening the rings. The amino acid analysis of CFG can be used to explain that the content of tyrosine, histidine and lysine obviously decreased after the reaction, and PGDE cross-linking occured at these sites. The chemical shift of C in tyrosine was observed in solid state 13C NMR spectra, so that it further proved the cross-linking PGDE with tyrosine residue. It is supposed that an intermolecular network structure can be formed by the cross-linking between PGDE and tyrosine, histidine and lysine residue of silk fibroin, which make CFG homogeneous and with better transparence and flexibility.

Key words: Silk fibroin, Gel, Chemical cross-linking, Epoxide

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