高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (10): 1855-1857.

• 研究简报 • 上一篇    下一篇

尿毒症毒素多肽二级结构的测定和预测

李国华1,2, 李纪红1, 杨眉1, 王蔚1, 孙平川1, 袁直1   

  1. 1. 南开大学高分子化学研究所,天津300071;
    2. 河北工业大学化工学院制药工程系,天津300130
  • 收稿日期:2004-10-15 出版日期:2005-10-10 发布日期:2005-10-10
  • 基金资助:

    国家“九七三”计划项目(批准号:G1999064707);国家自然科学基金(批准号:59873011)资助.

Determination and Forecast of Secondary Structure of Uremic Toxic Polypeptide

LI Guo-Hua1,2, LI Ji-Hong1, YANG Mei1, WANG Wei1, SUN Ping-Chuan1, YUAN Zhi1   

  1. Institute of Polymer Chemistry,Nankai University,Tianjin 300071,China
  • Received:2004-10-15 Online:2005-10-10 Published:2005-10-10

关键词: 尿毒症毒素多肽, 次级化学位移, 线性聚合物

Abstract: A uremic toxic compound with molecular weight 1007.94 was determined to be an octapeptide by mass spectrometry.Its amino-acid sequence was given as follos: Val-Val-Arg-Gly-Cys-Thr-Trp-Trp.Spin systems for amino acid residues in the octapeptide were identified through analysis of 2D NMR 1H-1H DQF-COSY,TOCSY and ROESY spectra acquired in H2O and D2O.Moreover,the complete assignment of proton resonances for the backbone and side chain was achieved.Based on the secondary chemical shift(Δδ) of the residues,the secondary structure of octapeptide was surveyed.Conformational analyses according to Chemical Shift Index(CSI) showed that the secondary structure of the octapeptide was principally α-helix.The CD spectra of the peptide in aqueous solution gave the same result.Additions of linear polymers made the conformations of octapeptide stretch.These experimental results provide a basis for further comprehension of interaction regulation between biomacromolecule and polymer absorbing materials.

Key words: Uremic toxic polypeptide, Secondary chemical shift, Lina polymer

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