高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (10): 1832-1834.

• 研究简报 • 上一篇    下一篇

一种新型超临界流体萃取-高效液相色谱在线联用系统的构建

张洁1, 段继诚1, 梁振1, 张丽华1, 张维冰1, 霍玉书2, 张玉奎1   

  1. 1. 中国科学院大连化学物理研究所,国家色谱研究分析中心,大连116023;
    2. 德克萨斯大学健康科学中心,圣安东尼奥7822923900
  • 收稿日期:2005-01-27 出版日期:2005-10-10 发布日期:2005-10-10
  • 基金资助:

    中国科学院知识创新工程重要方向性项目(批准号:KGCX2-SW-213)资助.

Establishment of a New On-line Coupled Supercritical Fluid Extraction(SFE) with High Performance Liquid Chromatography(HPLC) System

ZHANG Jie1, DUAN Ji-Cheng1, LIANG Zhen1, ZHANG Li-Hua1, ZHANG Wei-Bing1, HUO Yu-Shu2, ZHANG Yu-Kui1   

  1. 1. National Chromatographic R.& A.Center,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China;
    2. Health Science Center at San Antonio,The University of Texas,San Antonio,TX 78229-3900,USA
  • Received:2005-01-27 Online:2005-10-10 Published:2005-10-10

关键词: 在线联用系统, 超临界流体萃取, 高效液相色谱, 灵芝

Abstract: A new on-line coupled supercritical fluid extraction(SFE) with high performance liquid chromatography(HPLC) system was developed.Two three-port valves were used as an interface to connect SFE and HPLC.In this design,the two important processes including sample preparation and sample analysis were(integrated) into a system.Compared with off-line system,the on-line system has many advantages,such as high sensitivity,short analysis time,avoiding sample pollution,easily operation and ease to automation etc.Its application in the extraction and analysis of triterpenoids to the fruiting bodies of Ganoderma Lucidum was also described.Under the optimized extraction conditions,the similar chromatogram of the extract with on-line SFE- HPLC system was observed as well as that with off-line SFE followed by HPLC.The result showed the great potential of the on-line system in the extraction and analysis of tradition Chinese medicine.

Key words: On-line coupled system, Supercritical fluid extraction, High performance chromatography, Ganoderma lucidum

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