高等学校化学学报

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (9): 1645.

• 研究简报 • 上一篇    下一篇

基于溶胶-凝胶壳聚糖/SiO2杂化材料的安培型葡萄糖生物传感器

谭学才1,2, 翟海云1, 李荫1, 邹小勇1,3, 蔡沛祥1   

  1. 1. 中山大学化学与化学工程学院, 广州510275;
    2. 广西民族学院化学与生态工程学院, 南宁530006;
    3. 湖南大学化学生物传感与计量学国家重点实验室, 长沙410082
  • 收稿日期:2004-01-14 出版日期:2004-09-24 发布日期:2004-09-24
  • 基金资助:

    广东省自然科学基金(批准号:011105,031577);广西壮族自治区自然科学基金(批准号:0141037,0342018)资助

Amperomertric Glucose Biosensor Based on Sol-gel Chitosan/Silica Hybrid Material

TAN Xue-Cai1,2, ZHAI Hai-Yun1, LI Yin1, ZOU Xiao-Yong1,3, CAI Pei-Xiang1   

  1. 1. School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China;
    2. College of Chemistry and Ecological Engineering, Guangxi University for Nationalities, Nanning 530006, China;
    3. State key laboratory of Chem/Biosensing and Chemomitrics, Hunan University, Changsha 410082, China
  • Received:2004-01-14 Online:2004-09-24 Published:2004-09-24

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关键词: 溶胶-凝胶, 壳聚糖/二氧化硅杂化材料, 葡萄糖, 生物传感器, 普鲁士蓝

Abstract: A new type of sol-gel/organic hybrid composite film based on chitosan(CS) with methytrimethoxysilane(MTOS) was developed for the fabrication of an amperometric glucose biosensor. The hybrid composite film was used to immobilize glucose oxidase(GOD) on the surface of Prussian Blue(PB)-modified glass carbon electrode. Effects of some experimental conditions such as pH, temperature and applied potential etc. on the current response of the biosensor were investigated. The biosensor has a fast response less than 10 s and linear calibration range from 5.0×10-6 to 2.4×10-3 mol/L with detection limit of 1.0×10-6 mol/L. The apparent Michaelis-Menten constant Km is found to be 3.2×10-3 mol/L. The activation energy for enzymatic reaction is calculated to be 21.9 kJ/mol. The biosensor has a high sensitivity(420 μA·mol-1·L), long-term stability and good selectivity. This method has been used to determine the content of glucose in the real blood samples.

Key words: Sol-gel, Chitosan/Silica hybrid composite material, Glucose biosensor, Prussian blue

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