高等学校化学学报

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (9): 1642.

• 研究快报 • 上一篇    下一篇

毛细管电泳免疫化学发光检测鼠血管平滑肌细胞中zmol骨形成蛋白-2

王军华1, 黄卫华1, 刘彦明2, 程介克1   

  1. 1. 武汉大学化学与分子科学学院, 武汉430072;
    2. 河南信阳师范学院化学化工学院, 信阳464000
  • 收稿日期:2004-03-04 出版日期:2004-09-24 发布日期:2004-09-24
  • 基金资助:

    国家自然科学基金(批准号:20299034)资助

apillary Electrophoresis Immunoassay Chemiluminescence Detection of zmol Bone Morphogenic Protein-2 in Rat Vascular Smooth Muscle Cells

WANG Jun-Hua1, HUANG Wei-Hua1, LIU Yan-Ming2, CHENG Jie-Ke1   

  1. 1. College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072, China;
    2. College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China
  • Received:2004-03-04 Online:2004-09-24 Published:2004-09-24

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被引次数

16

关键词: 毛细管电泳, 化学发光, 免疫分析, 抗原抗体, 骨形成蛋白-2

Abstract: In this study, a capillary electrophoresis immunoassay(CEIA) method based on the enhanced chemiluminescence(CL) detection was developed. A horseradish peroxidase(HRP) label catalyzing the luminol/H2O2/p-iodophenol(PIP) reaction was performed, and the HRP was detected with detection limit(S/N=3) of 4.4 pmol/L(53 zmol), which is one of the highest sensitivity of HRP reported yet. The HRP was linked to bone morphogenic protein-2(BMP-2) in rat vascular smooth muscle(VSM) cells in noncompetitive format and first detected by CL. HRP-Ab6-mAb-BMP-2 complexes were baseline separated from free HRP label in 3 min. The detection limit(S/N=3) of BMP-2 is 6.2 pmol/L(75 zmol). This technique has been applied to arteriosclerosis pathology research. The change of BMP-2 contentin VSM cells which were stimulated by angiotensin Ⅱ(AgⅡ) for different hours was investigated in the concentration range of 1.0-10.0 pmol/L. The results are in accord with that obtained by common used Pathology image analysis system.

Key words: Capillary electrophoresis, Chemiluminescence, Immunoassay, Antigen antibody, Bone morphogenic protein-2

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