高等学校化学学报

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (6): 1183.

• 研究简报 • 上一篇    下一篇

一种新型低密度脂蛋白吸附剂的制备

傅国旗1, 陈新福1, 袁直1, 刘斌2, 沈彬2, 何炳林1   

  1. 1. 南开大学高分子化学研究所, 吸附分离功能高分子材料国家重点实验室, 天津 300071;
    2. 天津高等医学专科学校, 天津 300052
  • 收稿日期:2003-04-30 出版日期:2004-06-24 发布日期:2004-06-24
  • 通讯作者: 傅国旗(1966年出生),男,博士,副教授,从事生物医用吸附材料及吸附基础和应用研究.E-mail:guoqi_fu@sohu.com E-mail:guoqi_fu@sohu.com
  • 基金资助:

    国家基础研究发展规划项目(批准号:G1999064707)资助

Preparation of a New Adsorbent for Removal of Low Density Lipoprotein

FU Guo-Qi1, CHEN Xin-Fu1, YUAN Zhi1, LIU Bin2, SHEN Bin2, HE Bing-Lin1   

  1. 1. State Key Laboratory of Functional Polymer Materials for Adsorption and Separation, Institute of Polymer Chemistry, Nankai University, Tianjin 300071, China;
    2. Tianjin Medical College, Tianjin 300052, China
  • Received:2003-04-30 Online:2004-06-24 Published:2004-06-24

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被引次数

78

摘要： 研究表明,人体血浆中低密度脂蛋白(LDL)的含量过高会引起动脉硬化,进而诱发各种心脑血管疾病[1].近年来,采用血液净化吸附剂去除LDL受到了广泛的关注[2～10].目前临床上使用的吸附剂主要有固载LDL抗体的琼脂糖吸附剂及固载肝素或磺化葡聚糖的纤维素吸附剂,它们虽有较好的吸附效果,但由于存在配基昂贵、吸附剂成本高及机械强度差等问题,这些吸附剂的应用受到了很大的限制.本文以具有良好机械强度和血液相容性的聚乙烯醇(PVA)大孔珠状树脂为载体,以廉价易得的吲哚-3-乙酸(IAA)为配基,分别以不同长度的多胺为悬臂,制备了一种新型LDL吸附剂,并初步考察了其对LDL的吸附性能.

关键词: 低密度脂蛋白, 吸附, 吲哚-3-乙酸, 色氨酸, 大孔聚乙烯醇树脂, 聚乙烯醇, 磺化, 悬臂, 低密度脂蛋白

Abstract: On the basis of low density lipoprotein (LDL) adsorption on matrix-bound tryptophan,a new LDL adsorbent was prepared with indole-3-acetic acid (IAA) as a ligand,which had indole group in its chemical structure just as tryptophan. Macroporous polyvinyl alcohol (PVA) beads were obtained by suspension copolymerization of vinyl acetate and triallyl isocyanurate in the presence of porogen,followed by subsequent alcoholysis step. The PVA beads were allowed to react with epichlorohydrin,and the thus obtained epoxidized beads were then reacted with several polyamines to introduce space arms with different lengths. IAA was then coupled to the spacer-attaching beads by using DCC/HOBt method employed usually in polypeptide synthesis. The primary in vitro adsorption tests show that the resultant adsorbents provide a satisfactory binding capacity for LDL,and manifest much larger capacity than the adsorbent prepared by direct immobilization of tryptophan on the epoxy-activated PVA beads. This proves that indole groups do play an important part in binding LDL.

Key words: Low density lipoprotein, Adsorption, Indole-3-acetic acid, Tryptophan, Macroporous PVA beads, Poly(vinyl alcohol), Sulfonation, Spacer arm, Low density lipoprotein

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