兽疫链球菌变异株产生的透明质酸的纯化及表征

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (5): 853.

兽疫链球菌变异株产生的透明质酸的纯化及表征

洪水声¹, 陈佳¹, 滕利荣¹, 孟庆繁¹, 张建国², 刘兰英¹

1. 吉林大学生命科学学院, 长春 130023;
2. 中国科学院长春应用化学研究所电分析化学国家重点实验室, 长春 130022

收稿日期:2003-06-05 出版日期:2004-05-24 发布日期:2004-05-24
通讯作者: 刘兰英(1942年出生),女,教授,博士生导师,从事酶和多糖的研究.E-mail:ying@public.cc.jl.cn E-mail:ying@public.cc.jl.cn
基金资助:
国家高等教育科学“十五”规划项目(批准号:2002,20-58-47);吉林省教育科学十五规划重点项目(批准号:2001-11-015338)资助

Purification and Structure Characterization of the Hyaluronic Acid Produced by a Streptococcus Zooepidemicus Mutation Strain

HONG Shui-Sheng¹, CHEN Jia¹, TENG Li-Rong¹, MENG Qing-Fan¹, ZHANG Jian-Guo², LIU Lan-Ying¹

1. College of Life Science, Jilin University, Changchun 130023;
2. State Key Laboratory of Electroanalytical Chemistry, Chinese Acadamy of Sciences, Changchun Institute of Applied Chemistry, Changchun 130022, China

Received:2003-06-05 Online:2004-05-24 Published:2004-05-24

摘要/Abstract

摘要： 用N-甲基-N′-硝基-N-亚硝基胍(NTG)对兽疫链球菌进行诱变,获得高产菌株.经过对该菌株的发酵培养,将产生的多糖经Savage法、季铵复合物沉淀法、DEAE-纤维素(DE52)离子交换层析法及SephadexG-75凝胶过滤法分离纯化.纯化的多糖结构经化学组成分析、核磁共振波谱、红外光谱及圆二色谱鉴定,证明了诱变得到的高产菌株(StreptococcuszooepidemicusJ18)再经发酵,得到的多糖为透明质酸.通过刚果红实验证明了透明质酸的构象为单股螺旋结构,其平均分子量约为1.16×10⁶.

关键词: 兽疫链球菌变异株, 透明质酸, 分离纯化, 表征

Abstract: A mutated strain of Streptococcus zooepidemicus capable of producing the polysaccharide in a high yield was obtained after being mutated bythe chemical agent N-methyl-N′-nitro-N- nitrosoguanidine(NTG). Polysaccharide produced bythe mutated strain of Streptococcus zooepidemicus was purified bythe procedure including Savage method, quaternary ammonium compound precipitation, DEAE-cellulose(DE52) column chromatograph and Sephadex G-75 gel filtration. The purified polysaccharide was proved to be hyaluronic acid(HA) by means of chemical composition analysis, ¹³C NMR, Infrared spectra, and circular dichroism. The single helix conformation of the purified HA was studied by Congo red experiment. The molecular weight of the HA was about 1.16×10⁶, which was measured by viscosity method.

Key words: Streptococcus zooepidemicus, Hyaluronic acid, Separation and purification, Characterization

中图分类号:

O657

TrendMD:

洪水声, 陈佳, 滕利荣, 孟庆繁, 张建国, 刘兰英. 兽疫链球菌变异株产生的透明质酸的纯化及表征. 高等学校化学学报, 2004, 25(5): 853.

HONG Shui-Sheng, CHEN Jia, TENG Li-Rong, MENG Qing-Fan, ZHANG Jian-Guo, LIU Lan-Ying . Purification and Structure Characterization of the Hyaluronic Acid Produced by a Streptococcus Zooepidemicus Mutation Strain. Chem. J. Chinese Universities, 2004, 25(5): 853.

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