高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (12): 2256.

• 研究简报 • 上一篇    下一篇

毛细管电泳高频电导法测定青风藤药材及制剂中青藤碱的含量

翟海云1, 王峻梅2, 陈缵光2, 徐健君1, 蔡沛祥1, 莫金垣1   

  1. 1. 中山大学化学与化学工程学院, 广州510275;
    2. 中山大学药学院, 广州510089
  • 收稿日期:2004-01-07 出版日期:2004-12-24 发布日期:2004-12-24
  • 基金资助:

    国家自然科学基金(批准号:20375049);广东省自然科学基金重点项目(批准号:021808)资助

Determination of Sinomenine in Sinomenium acutum and Sinomenium Tablets by Capillary Electrophoresis with High Frequency Conductivity Detector

ZHAI Hai-Yun1, WANG Jun-Mei2, CHEN Zuan-Guang XU Jian-Jun2, CAI Pei-Xiang1, MO Jin-Yuan1   

  1. 1. College of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China;
    2. School of Pharamaceutical Sciences, Sun Yat-sen University, Guangzhou 510089, China
  • Received:2004-01-07 Online:2004-12-24 Published:2004-12-24

关键词: 毛细管电泳, 高频电导检测, 非接触式电导检测, 青藤碱, 青风藤

Abstract: A method based on capillary electrophoresis with a high frequency conductivity detection was developed for the determination of Sinomenine. The key factors for separation and determination were studied and the best analysis conditions were obtained. In the buffer of 2.0 mmol/L HAc-3.0 mmol/L NaAc-10.0% CH3OH at a separation voltage of 22 kV, Sinomenine in the sample could be separated and detected within 4 min. The linear mass concentration of Sinomenine is in the range of 1.0-36.0 μg/mL, the limit of detection reached 0.20 μg/mL. The method was used for the analysis of Sinomenine in Sinomenium acutum and tablets satisfactorily with a recovery of 96%-102%.

Key words: Capillary electrophoresis, High frequency conductivity detection, Contactless conductivity detection, Sinomenine, Sinomenium acutum

中图分类号: 

TrendMD: