高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (11): 2001-2003.

• 研究简报 • 上一篇    下一篇

手性钌(Ⅱ)配合物对DNA的立体选择性断裂

王芳1, 张黔玲1, 刘剑洪1, 张培新1, 刘毅3, 任祥忠1, 刘建忠2, 计亮年2   

  1. 1. 深圳大学师范学院化学与生物学系, 深圳518060;
    2. 中山大学教育部基因工程重点实验室, 广州510275;
    3. 深圳大学师范学院物理系, 深圳518060
  • 收稿日期:2003-12-12 出版日期:2004-11-24 发布日期:2004-11-24
  • 基金资助:

    广东省自然科学基金(批准号:04300882);深圳市科学技术计划基金项目(批准号:200328)资助

Stereoselective Cleavage of DNA by Chiral Ruthenium(Ⅱ) Complexes

WANG Fang1, ZHANG Qian-Ling1, LIU Jian-Hong1, ZHANG Pei-Xin1, LIU Yi3, REN Xiang-Zhong1, LIU Jian-Zhong2, JI Liang-Nian2   

  1. 1. Department of Chemistry and Biology, Normal College, Shenzhen University, Shenzhen 518060, China;
    2. Key Laboratory of Gene Engineering of Ministry of Education, Zhongshan University, Guangzhou 510275, China;
    3. Department of Physics, Normal College, Shenzhen University, Shenzhen 518060, China
  • Received:2003-12-12 Online:2004-11-24 Published:2004-11-24

关键词: 手性钌(Ⅱ)配合物, DNA, 插入, 断裂

Abstract: Two enantiomerically pure polypyridyl ruthenium(Ⅱ) complexes Δ- and Λ-[Ru(bpy)2HPIP](PF6)2{HPIP=2-(2-hydroxyphenyl)imidazo[4,5-f][1,10]phenanthroline} were synthesized and characterized. DNA-binding studies indicated that both enantiomers bound to calf thymus DNA by intercalation, the Δ- enantiomer exhibited a stronger binding affinity than the Λ- enantiomer. Upon irradiation at 302 nm, both enantiomers were found to promote cleavage of plasmid pBR322 DNA from the supercoiled form Ⅰ to the open circular form Ⅱ, but the Δ-enantiomer exhibited a higher cleaving efficiency for DNA due to the different binding affinities to DNA. The cleaving mechanisms for Δ- and Λ-[Ru(bpy)2HPIP]2+ were identical, the hydroxyl radical(OH·) was likely to be the reactive specie responsible for the cleavage of plasmid pBR322, and the photoreduction of Ru(Ⅱ) complex with concomitant hydroxide oxidation was the important step in the DNA cleavage reaction.

Key words: Chiral ruthenium(Ⅱ) complex, DNA, Intercalation, Cleavage

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