微流控芯片用于流式细胞术的基础研究

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (10): 1816.

微流控芯片用于流式细胞术的基础研究

杨蕊¹, 牟颖^1,2, 邹明强^1,3, 吴喆², 金钦汉¹

1. 吉林大学化学学院微分析仪器研究所, 吉林省光谱分析仪器工程技术研究中心;
2. 吉林大学分子酶学工程教育部重点实验室, 长春130023;
3. 吉林出入境检验检疫局, 长春130062

收稿日期:2004-03-04 出版日期:2004-10-24 发布日期:2004-10-24
基金资助:
国家质量检验总局重大科学技术攻关项目(批准号:2003IK057)资助

Studies on the Method of Flow Cytometry Integrated with Microfluidic Chip

YANG Rui¹, MU Ying^1,2, ZOU Ming-Qiang^1,3, WU Zhe², JIN Qin-Han¹

1. Institute for Miniature Instrumentation, College of Chemistry; Jilin Province Research Center for Engineering Technology of Spectral Instruments, Jilin University, Changchun 130023, China;
2. Key Laboratory for Molecular Enzymology Engineering of Ministry of Education, Jilin University, Changchun 130023, China;
3. Jilin Entry-Exit Inspection and Quarantine Bureau, Changchun 130062, China

Received:2004-03-04 Online:2004-10-24 Published:2004-10-24

摘要/Abstract

摘要： 自行设计并加工了玻璃微流控芯片,并将其与流式细胞术相结合,采用羟丙基甲基纤维素(HPMC)的磷酸盐溶液为缓冲体系和鞘液,解决了微粒在微芯片中流动的若干问题,使其状态可以得到更有效的控制.采用自行组装的激光诱导荧光装置并结合动电聚焦技术,实现了对荧光微球的计数,并可通过荧光倒置显微镜实时观察到微通道内微球的实际流动情况.方法简单,操作方便,并且具有仪器体积小、试剂及样品用量少和分析速度快等优点.

关键词: 微流控芯片, 荧光微球, 流式细胞术, 动电聚焦, 吸附

Abstract: A microfluidic chip is designed and fabricated, and coupled with flow cytometry. By using Na₂HPO₄ solution added with hydroxypropyl methyl cellulose(HPMC) as buffer and sheathing fluid, the problems met with the flowing of microspheres in the microchannel were solved, and the fluidic conditions were under more effective control. By using a home-made laser induced fluorescence system and electrokinetic focusing, the counting of the microspheres and real time observation of their actual fluidic state through an inverted fluorescence microscope is made. The whole system is compact and easy to be operated. The comsumption of reagent and sample is little and the speed of the assay is quick.

Key words: Microfluidic chip, Fluorescence microspheres, Flow cytometry, Electrokinetic focusing, Adsorption

中图分类号:

O657

TrendMD:

杨蕊, 牟颖, 邹明强, 吴喆, 金钦汉. 微流控芯片用于流式细胞术的基础研究. 高等学校化学学报, 2004, 25(10): 1816.

YANG Rui, MU Ying, ZOU Ming-Qiang, WU Zhe, JIN Qin-Han . Studies on the Method of Flow Cytometry Integrated with Microfluidic Chip. Chem. J. Chinese Universities, 2004, 25(10): 1816.

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