柔红霉素与DNA作用的序列特异性研究

高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (8): 1395.

柔红霉素与DNA作用的序列特异性研究

程圭芳, 张冬梅, 丁敏, 屈海云, 何品刚, 方禹之

华东师范大学化学系, 上海 200062

收稿日期:2002-03-06 出版日期:2003-08-24 发布日期:2003-08-24
通讯作者: 方禹之(1931年出生),男,教授,博士生导师,从事生物电化学、光谱电化学和毛细管电泳等方面的研究.
基金资助:
国家自然科学基金(批准号:29875008)资助

Studies on the Interaction of Daunomycin and the Specific Sequence of DNA

CHENG Gui-Fang, ZHANG Dong-Mei, DING Min, QU Hai-Yun, HE Pin-Gang, FANG Yu-Zhi

Department of Chemistry, East China Normal University, Shanghai 200062, China

Received:2002-03-06 Online:2003-08-24 Published:2003-08-24

摘要/Abstract

摘要： 采用紫外-可见光谱法和紫外-可见光谱电化学法研究了柔红霉素(DNR)与不同寡聚核苷酸之间的相互作用.结果表明,DNR优先作用于寡聚核苷酸的CpG位点,然后是ApG和ApC.因为DNR可与鸟嘌呤之间形成3个氢键.与双链寡聚核苷酸作用时,DNR最先插入的位点是(CpG)₂碱基对之间,其次是(TpG)(CpA)和(GpC)(ApC)碱基对之间.当DNR与存在未配对G碱基的DNA链作用时,因游离的DNR量增加使其电化学活性增加,导致DNA构象和构型的变化,使DNA生理功能受到损伤,DNA碱基增色效应显著上升.此法可用于G碱基未配对DNA链的检测.

关键词: 柔红霉素, 蒽环类抗癌药物, DNA, 序列特异性, 光谱电化学

Abstract: On the ground of UV-spectrophotometry and UV-spectroelectrochemistry, the interaction of daunomycin(DNR) and specific sequence of DNAhas been investigated.As the results obtained in this paper, DNRcan react with the sequence of oligonucleotide which is CpG, ApGand ApCsuccessively; the sequence of base pairs of double helix of DNAwhich are intercalated by DNRare (CpG)₂, (TpG)(CpA) and (CpT)(ApG).An important conclusion has been gained in this paper that if Gbases were not pairing in the helix of DNA, the spectroelectrochemical characteristic of the adducts containing that kind of DNAand DNRwould exhibit a remarkable difference with those adducts consisting of DNRand normal DNA.It indicates that this characteristic can be used to detect and determine the DNAhelix which has one base unpaired or mispairing.The results obtained in this paper are of clinical and pharmacaLImportance.

Key words: Daunomycin, Anathracycline, DNA Speciality, Spectroelectrochemistry

中图分类号:

O657

TrendMD:

程圭芳, 张冬梅, 丁敏, 屈海云, 何品刚, 方禹之. 柔红霉素与DNA作用的序列特异性研究. 高等学校化学学报, 2003, 24(8): 1395.

CHENG Gui-Fang, ZHANG Dong-Mei, DING Min, QU Hai-Yun, HE Pin-Gang, FANG Yu-Zhi . Studies on the Interaction of Daunomycin and the Specific Sequence of DNA. Chem. J. Chinese Universities, 2003, 24(8): 1395.

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