高等学校化学学报

高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (3): 404.

• 论文 • 上一篇    下一篇

基于拮抗作用检测除草剂的类囊体膜生物传感器研究

李建平1,2, 彭图治1, 贺筱蓉3, 肖海军3   

  1. 1. 浙江大学西溪校区化学系, 杭州 310028;
    2. 桂林工学院应用化学系, 桂林 541004;
    3. 浙江大学生物系, 杭州 310028
  • 收稿日期:2002-05-29 出版日期:2003-03-24 发布日期:2003-03-24
  • 通讯作者: 彭图治(1946年出生),男,教授,博士生导师,主要从事电分析化学和生物分析化学研究.E-mail:tzp@emb.zju.edu.cn E-mail:tzp@emb.zju.edu.cn
  • 基金资助:

    浙江省自然科学基金(批准号:200061);分析测试基金资助

A Thylakoid Membrane Biosensor Based on the Antagonism of Thylakoid-bound Enzymes for Determining Herbicide

LI Jian-Ping1,2, PENG Tu-Zhi1, HE Xiao-Rong3, XIAO Hai-Jun3   

  1. 1. Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China;
    2. Department of Applied Chemistry, Guilin Institute of Technology, Guilin 541004, China;
    3. Department of Biology, Zhejiang University, Hangzhou 310028, China
  • Received:2002-05-29 Online:2003-03-24 Published:2003-03-24

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被引次数

46

摘要： 利用除草剂对植物类囊体束缚酶分解过氧化氢的拮抗作用,研制了一种快速检测痕量除草剂的电化学生物传感器.将植物类囊体用聚乙烯醇-苯乙烯吡啶(PVA-SbQ)光敏聚合剂在紫外光诱导下产生大分子网状结构进行包埋,制成生物敏感膜,并固定在铂电极表面.根据加入除草剂时类囊体膜束缚酶分解过氧化氢活性的变化,对除草剂进行测定.在含有1×10-3mol/LNaCl,5×10-3mol/LMgCl2和0.01mol/LH2O2的Tris-HCl缓冲溶液(pH=7.4)中,基于测量0.65V处H2O2氧化电流的变化,可以对下列浓度的除草剂进行定量检测:百草枯3×10-9～1.5×10-7mol/L,敌草龙1×10-8～3×10-7mol/L,扑草净4×10-8～3×10-6mol/L,阿特拉津1×10-7～5×10-6mol/L,莠灭净1×10-7～5×10-6mol/L.利用PVA-SbQ光聚合膜固定类囊体,能够使酶的活性在低温下保持数月.

关键词: 类囊体, 拮抗作用, 除草剂, 生物传感器, 示差脉冲伏安法

Abstract: An electrochemical biosensor for determination of herbicide was prepared. It was based on the antagonism of herbicides on the decomposition of hydrogen peroxide catalyzed by the thykaloid-bound enzymes. The thylakoid membrane isolated from spinach leaves was immobilized by entrapment in polyvinylalcohol bearing styrylpyridinium groups(PVA-SbQ). The thylakoid membrane was fixed on the head of a platinum electrode. Oxidative current of hydrogen peroxide was detected by differential pulse voltammetry in Tris-HCl buffer solution(pH=7.4) containing 1×10-3 mol/L NaCl, 5×10-3 mol/L MgCl2 and 0.01 mol/L hydrogen peroxides. Some herbicides have been detected in the concentration ranges of 3×10-9-1.5×10-7 mol/L for paraquat, 1×10-8-3×10-7 mol/L for diuron, 4×10-8-3×10-6 mol/L for prometryn, 1×10-7- 5×10-6 mol/L for atrazine and 1×10-7- 5×10-6 mol/L for ametryn, respectively. The method for immobilizing thylakoid in PVA-SbQ membrane was found to be promising to make enzymes stable for several months. The mechanism for this antagonism on the enzymatic decomposition of hydrogen peroxide has been discussed.

Key words: Thylakoid, Antagonism, Herbicide, Biosensor, Differential pulse voltammetry

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