高等学校化学学报

高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (12): 2165.

• 论文 • 上一篇    下一篇

N-(2-甲基-6-乙基苯基)丙氨酸对映体的毛细管电泳分离

郑良玉1,2, 张锁秦3, 高贵1, 曹淑桂1, 张桂荣1, 王智1, 韩四平1, 权静1   

  1. 1. 吉林大学分子酶学工程教育部重点实验室;
    2. 吉林大学生命科学学院;
    3. 吉林大学化学学院, 长春 130023
  • 收稿日期:2002-12-24 出版日期:2003-12-24 发布日期:2003-12-24
  • 通讯作者: 曹淑桂(1944年出生),女,教授,博士生导师,主要从事非水酶学和手性拆分研究.E-mail:sgcao@public.cc.jl.cn E-mail:sgcao@public.cc.jl.cn
  • 基金资助:

    国家自然科学基金(批准号:20272017)资助

Separation of N-(2-Ethyl-6-methylphenyl)alanine Enantiomers by Capillary Electrophoresis

ZHENG Liang-Yu1,2, ZHANG Suo-Qin3, GAO Gui1, CAO Shu-Gui1, ZHANG Gui-Rong1, WANG Zhi1, HAN Si-Ping1, QUAN Jing1   

  1. 1. Key Laboratory of Molecular Enzymology and Engineering of Educational Ministry;
    2. College of Life Science;
    3. College of Chemistry, Jilin University, Changchun 130023, China
  • Received:2002-12-24 Online:2003-12-24 Published:2003-12-24

PDF (PC)

被引次数

10

摘要： 采用高效毛细管区带电泳法,以β-环糊精及其衍生物作为手性选择剂,对外消旋N-(2-甲基-6-乙基苯基)丙氨酸(EMPA)的两个对映体进行了手性分离,比较了环糊精种类、环糊精浓度、电解质溶液pH值、温度和电场强度对分离的影响.实验结果表明,采用2,6-O-二甲基-β-环糊精为手性选择试剂,环糊精浓度为40mmol/L、电解质溶液pH=5.5及温度为20℃时分离效果最佳,对映体基本达基线分离,线性范围为20～200mg/L,最低检测限为10mg/L.

关键词: 毛细管电泳, 手性分离, N-(2-甲基-6-乙基苯基)丙氨酸

Abstract: Capillary electrophoresis was used for the chiral separation of N-(2-ethyl-6-methylphenyl)alanine enantiomers in this paper. Capillary zone electrophoresis conditions were used with β-cyclodextrin or its derivatives as a chiral mobile phase additive. The effects of cyclodextrin type, variation of cyclodextrin concentration, background electrolyte pH, temperature and field strength were investigated. Optimum separation was achieved for N-(2-ethyl-6-methylphenyl)alanine enantiomers by using 2,6-di-O-methyl-β-cyclodextrin(40 mmol/L), the temperature(20 ℃) and pH(5.5). The mass concentration range from 20 to 200 mg/Lproved to be sufficient for exact quantification. The detection limitation is 10 mg/L. Anew method of using capillary electrophoresis for the chiral separation of N-(2-ethyl-6-methylphenyl)alanine enantiomers has been established.

Key words: Capillary electrophoresis, Chiral separation, N-(2-Ethyl-6-methylphenyl)alanine

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