高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (10): 1753.

• 研究简报 • 上一篇    下一篇

新型多吡啶配体的设计及其对配合物与DNA作用机制的影响

张黔玲1, 刘剑洪1, 刘建忠2, 任祥忠1, 张培新1, 魏波1, 徐宏1, 计亮年2   

  1. 1. 深圳大学师范学院化学与生物学系, 深圳 518060;
    2. 中山大学化学与化工学院, 广州 510275
  • 收稿日期:2002-11-28 出版日期:2003-10-24 发布日期:2003-10-24
  • 通讯作者: 刘剑洪(1964年出生),男,教授,从事生物及高分子材料研究.E-mail:liujh@szu.edu.cn E-mail:liujh@szu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:29801005)资助

Design of New Polypyridyl Ligands and Their Effects on DNA-binding Mechanisms of Complexes

ZHANG Qian-Ling1, LIU Jian-Hong1, LIU Jian-Zhong2, REN Xiang-Zhong1, ZHANG Pei-Xin1, WEI Bo1, XU Hong1, JI Liang-Nian2   

  1. 1. Department of Chemistry and Biology, Normal College, Shenzhen University, Shenzhen 518060, China;
    2. School of Chemistry and Engineering, Zhongshan University, Guangzhou 510275, China
  • Received:2002-11-28 Online:2003-10-24 Published:2003-10-24

关键词: 多吡啶钌配合物, DNA, 插入作用, 沟面结合

Abstract: Two structurally related polypyridyl ligands ODHIP(3,4-dihydroxyl-imidazophenanthroline), MDHIP(2,4-dihydroxyl-imidazo phenanthroline) and their ruthenium(II) complexes [Ru(phen)2ODHIP]2+and [Ru(phen)2MDHIP]2+were prepared and characterized.Their DNA-binding properties were studied by spectroscopic methods and viscosity measurements.The results indicated that the two complexes are bound to DNA by different modes due to the different planarities of ligands.For the complex [Ru(phen)2MDHIP] 2+, the 2-position ortho group of MDHIP could form an intramolecular hydrogen bond with the nitrogen atom of the imidazole ring to extend the planarity and strengthen the binding affinity.On the other hand, the 4-position ortho group hindered the complex from intercalating into the base pairs of DNA, and finally, making the complex bind to DNA by a partial intercalative mode.However, for the complex [Ru(phen)2ODHIP]2+, there was no intramolecular hydrogen bond formed and the two ortho groups further increased the steric effect and decreased the binding affinity, thus making the complex bind to DNAby groove binding mode.

Key words: Polypyridyl ruthenium complex, DNA, Intercalation, Groove binding

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