高等学校化学学报

高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (9): 1680.

• 研究论文 • 上一篇    下一篇

红四氮唑作为电化学嵌合剂的核酸杂交生物传感器

程琼2, 彭图治1   

  1. 1. 浙江大学化学系, 西溪校区, 杭州 310028;
    2. 嘉兴学院化学工程系, 嘉兴 314001
  • 收稿日期:2001-08-16 出版日期:2002-09-24 发布日期:2002-09-24
  • 通讯作者: 彭图治(1946年出生),男,教授,博士生导师,从事电分析化学和生物分析化学方面的研究.E-mail:tzp@emb.zju.edu.cn E-mail:tzp@emb.zju.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:29975024);浙江省自然科学基金(批准号:200061)资助

A Biosensor for Nucleic Acid Hybridization Using 2,3,5-Triphenyl-tetrazoliam Chloride as Electrochemical Intercalator

CHENG Qiong2, PENG Tu-Zhi1   

  1. 1. Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China;
    2. Department of Chemical Engineering, Jiaxing College, Jiaxing 314001, China
  • Received:2001-08-16 Online:2002-09-24 Published:2002-09-24

PDF (PC)

被引次数

30

摘要： 提出了一种以红四氮唑(TTC)作为嵌合剂,采用石墨修饰电极监测核酸杂交过程和测定特定DNA片段的灵敏的电化学方法.TTC具有背景电流低,电活性强和对双链DNA(dsDNA)选择性好等一系列优点,可在石墨电极表面形成dsDNA-TTC层.在电化学测定中,TTC的还原电流与靶DNA浓度具有良好的线性关系,对DNA的检测限可低达6×10-11mol/L.

关键词: DNA, 红四氮唑, 杂交, 伏安法

Abstract: Asensitive electrochemical biosensor is reported for the detection of nucleic acid hybridization and sequence-specific DNAs. The biosensor is based on a graphite electrode modified ssDNAusing 2,3,5-triphenyl-tetrazolium chloride(TTC) as hybridization indicator. TTCshows high electroactivity and low background current in electrochemical measurements. It binds to dsDNAs on the electrode surface with high selectivity. The reductive current of intercalated TTCis proportional to the concentration of target ssDNAin the range of 6.0×10-6—2.0×10-8 mol/Lin cyclic voltammetry. The detection limit of the biosensor for DNAis 6.0×10-11 mol/Lin differential pulse voltammetry.

Key words: DNA, 2,3,5-Triphenyl-tetrazolium chloride, Hybridization, Voltammetry

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