高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (9): 1676.

• 研究论文 • 上一篇    下一篇

表面增强拉曼光谱研究小檗碱与DNA的相互作用

王树玲, 于俊生   

  1. 南京大学化学系, 南京 210093
  • 收稿日期:2001-09-18 出版日期:2002-09-24 发布日期:2002-09-24
  • 通讯作者: 于俊生(1953年出生),男,博士,副教授,从事分子光谱及分析研究.E-mail:ndchemyu@public1.ptt.js.cn E-mail:ndchemyu@public1.ptt.js.cn
  • 基金资助:

    南京大学"九八五"学科发展基金(批准号:2000)资助

SERS Studies on the Interaction of Berberine With ct DNA

WANG Shu-Ling, YU Jun-Sheng   

  1. Department of Chemistry, Nanjing University, Nanjing 210093, China
  • Received:2001-09-18 Online:2002-09-24 Published:2002-09-24

摘要: 用表面增强拉曼光谱研究了小檗碱(BER)与小牛胸腺DNA(ctDNA)的相互作用,并对重要谱峰进行了归属.在Ag胶体系中,小檗碱分子的拉曼信号增强显著,表明小檗碱阳离子键合到银胶粒子的表面.加入ctDNA之后,小檗碱分子的大部分SERS带的强度进一步增加,而仅有少数几个带的信号强度趋于消失,可能是小檗碱分子的异喹啉部分键合到DNA的小沟槽,小檗碱与DNA的相互作用模式主要是通过静电力及疏水相互作用.吸收光谱表明,Ag胶体系的存在并未改变小檗碱分子与DNA的相互作用模式.

关键词: 表面增强拉曼光谱, 小檗碱, ctDNA

Abstract: The interaction between berberine and ct DNAwas investigated by using FT-Surface Enhancement Raman Scattering(SERS) spectroscopy. The FT-SERSspectra of berberine and its complex with DNAwere recorded in the Ag sol and pH7.20 Tris solution. Vibrational assignment of the SERSactive modes was done on the basis of normal coordinate analysis. Some Raman bands of berberine are dramatically increased in intensity on the silver colloid. Results suggest that berberine ring system around the nitrogen positively charged center should assume the perpendicular or at least tilted stances with respect to the surface of Ag sol nanoparticle through the electrostatic interaction. When ct DNAis added into the berberine solution, much stronger SERSsignal with a factor of 1.5 was obtained for the most of FT-SERSbands of the molecule due to DNAbinding; only a few bands approach to disappear in intensity. This result implies that there are two binding modes between berberine and double helix DNA. The shifts of some Raman band were also observed on the DNAbinding. SERSexperiments show that the isoquinolone moiety of berberine molecule may be accommodated in the minor groove of DNAhelix. Grooving binding is very likely to be the dominant mode in the interaction of berberine with double helix DNA.

Key words: Surface-enhanced Raman spectroscopy, Berberine, ct DNA

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