高等学校化学学报

高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (7): 1379.

• 研究论文 • 上一篇    下一篇

温度及药物诱导肿瘤细胞凋亡的实验观察

李莉1,2, 张珈敏1, 胡远扬1, 冯英2, 屈松生2   

  1. 1. 武汉大学生命科学院结构病毒室;
    2. 武汉大学化学与分子科学学院, 武汉 430072
  • 收稿日期:2000-08-09 出版日期:2002-07-24 发布日期:2002-07-24
  • 通讯作者: 胡远扬(1949年出生),男,教授,博士生导师,从事病毒学及生物超微病理学研究.
  • 基金资助:

    国家自然科学基金(批准号:29773033)

Experimentation on Induction of Tumor Cell Apoptosis by Temperature and Anti-tumor Medicine

LI Li1,2, ZHANG Jia-Min1, HU Yuan-Yang1, FENG Ying2, QU Song-Sheng2   

  1. 1. Laboratory of Structural Virus, College of Life Sciences;
    2. College of Chemistry and Molecule Science, Wuhan University, Wuhan 430072, China
  • Received:2000-08-09 Online:2002-07-24 Published:2002-07-24

PDF (PC)

被引次数

10

摘要： 将微量热法和透射电镜技术相结合,研究了温度对人卵巢癌细胞代谢的影响,不同温度下癌细胞超微结构的变化和加抗肿瘤药物(嘧福绿)的协同作用.从宏观到微观,深入探讨了热疗与化疗协同作用对肿瘤细胞杀伤作用的机理.实验结果表明,在高温下细胞代谢降低,肿瘤细胞的形态发生一系列恶性变化,且高热可能诱导肿瘤细胞的凋亡;热疗加化疗的协同作用则能加速肿瘤细胞的凋亡,使细胞的能量代谢更低,随着作用时间的延长,最终导致细胞死亡.

关键词: 微量热法, 透射电镜, 高温, 肿瘤细胞, 凋亡

Abstract: Techniques from microcalorimetry and transmission electron microscopy(TEM) were employed to investigate the metabolic effects and the ultrastructural changes of human body carcinoma of ovary cell line(COC1) at different temperatures with and without synergetic effects of anti tumor medicine(HCFU). The mechanism of hyperthemia and chemotherapy in the treatment of the tumor cells was explored at both macroscopic and microscopic levels. The results showed that the metabolic activity of the tumor cells was reduced at high temperature and the cell morphology changed malignantly. High temperature might induce apoptosis of the tumor cells; and the combined effects of hyperthemia and chemotherapy could speed up the apoptosis and reduce the metabolic power of the tumor cells. Asustained longer time action could lead to ultimate death of the tumor cells.

Key words: Microcalorimetry, TEM, High temperature, Tumor cell, Apoptosis

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