快速线扫描拉曼成像技术在研究CaSki细胞凋亡过程中的应用

doi:10.7503/cjcu20160265

摘要/Abstract

摘要：

应用快速线扫描拉曼成像技术研究了在抗肿瘤药物顺铂诱导下宫颈癌CaSki细胞凋亡过程中细胞色素c、蛋白质和脂质等的时空变化规律. 结果表明, 细胞色素c与蛋白质的相对拉曼峰强可以反映细胞凋亡程度. 与常用的表征细胞活性的噻唑蓝(MTT)实验的统计方法相比, 利用拉曼成像技术可以更灵敏地观察到细胞凋亡早期生物活性分子的变化, 从而在单细胞水平检测细胞凋亡过程, 为解析药物与细胞的作用机制提供分子水平信息.

关键词: 宫颈癌, 细胞色素c, 凋亡, 拉曼光谱, 拉曼成像, 噻唑蓝法

Abstract:

Cisplatin-induced apoptosis of CaSki cell was studied via Raman imaging technique. Fast line-scanning Raman imaging method could reveal the high temporal and spatial information of biomolecules including cytochrome c, protein and lipid during the apoptosis process. The results indicate that the relative intensity of cytochrome c to protein(I₇₅₀/I₁₆₆₀) could reflect the stage of apoptosis. Compared with the traditional methylthiazoletetrazolium(MTT) method, Raman imaging could investigate the apoptosis of single living cell at a much higher temporal resolution and provide the molecular fingerprint information for revealing the cell-drug interaction.

Key words: Cervical cancer, Cytochrome c, Apoptosis, Raman spectroscopy, Raman imaging, Methylthiazoletetrazolium(MTT) method

中图分类号:

O657.37
O644

TrendMD:

张金亮, 马鑫, 徐梦溪, 宗铖, 任斌. 快速线扫描拉曼成像技术在研究CaSki细胞凋亡过程中的应用. 高等学校化学学报, 2016, 37(7): 1257.

ZHANG Jinliang, MA Xin, XU Mengxi, ZONG Cheng, REN Bin. Study on Apoptosis Process of CaSki via Fast Line-scanning Raman Imaging^†. Chem. J. Chinese Universities, 2016, 37(7): 1257.

图/表 4

Fig.1 Viability of CaSki cells after being treated with different concentrations of cisplatin for 24 h

Fig.2 Time-depended viability of CaSki cells after being treated with 4 mmol/L cisplatin

Fig.3 Time depended bright field images(A1—F1) and Raman images using raman peaks of cytochrome c(750 cm-1)(A2—F2), protein(1660 cm-1)(A3—F3), and lipid(2852 cm-1)(A4—F4) of cells after being treated with 4 mmol/L cisplatin for 0 h(A1—A4), 4 h(B1—B4), 8 h(C1—C4), 12 h(D1—D4), 24 h(E1—E4) and 48 h(F1—F4)

Fig.4 Intensity of 750 and 1660 cm-1 peaks and ratio of I750/I1660 of the cells after being treated with 4 mmol/L cisplatin for different time

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