高等学校化学学报

高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (12): 2250.

• 研究简报 • 上一篇    下一篇

高分辨阿达玛变换显微荧光图像分析(Ⅰ)——系统的成像能力及其在单个肿瘤细胞分析中的应用

唐宏武, 周锦松, 吴琼水, 陈观铨   

  1. 武汉大学化学与分子科学学院测试中心, 武汉430072
  • 收稿日期:2001-09-10 出版日期:2002-12-24 发布日期:2002-12-24
  • 通讯作者: 唐宏武(1969年出生),男,博士,副教授,主要从事分子光谱分析和光谱仪器研究.E-mail:hw tang@whu.edu.cn E-mail:hwtang@whu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:20075019);武汉市青年科技“晨光计划”项目(批准号:995004078G)资助

High-resolution Hadamard Transform Microscopic Fluorescence Image Analysis(Ⅰ)──Imaging Capability and Quantitative Analysis on Single Tumor Cells

TANG Hong-Wu, ZHOU Jin-Song, WU Qiong-Shui, CHEN Guan-Quan   

  1. Center of Analysis and Measurements, College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072, China
  • Received:2001-09-10 Online:2002-12-24 Published:2002-12-24

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关键词: 阿达玛变换, 荧光成像, 显微术, 乳腺肿瘤, DNA倍性

Abstract: The imaging capability(sensitivity, signal-to-noise ratio, imaging speed and spatial resolution) of a domestic-made high-resolution Hadamrd transform(HT) microscopic fluorescence imaging system was discussed. The results show that the system can be applied for high-resolution single-cell imaging sensitively in a short time, the cell image provided by the system has a high-resolution up to 511×512 pixel. The results also shows that this system has a more excellent resolving power than conventional optical microscope, under a 100× objective(oil lens) the spatial resolution up to 0.24 μm per pixel in X direction was obtained, which is close to the resolution limit of conventional optical microscope. The system was used for multi-parameter evaluation of breast tumor malignancy and malignant grade for a single tumor cell based on nuclei DNA ploidy measurements, and satisfactory results were obtained.

Key words: Hadamard transform, Fluorescence imaging, Microscopy, Breast tumor, DNA ploidy

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