高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (7): 1117.

• 研究简报 • 上一篇    下一篇

肥皂草素Saporin-S6二级结构的圆二色光谱及激光喇曼光谱法表征

王永亭1, 黄品伟2, 李正强2, 徐菲1, 孟庆繁1, 杨谊1, 史学惠1, 李青山1   

  1. 1. 吉林大学生命科学学院, 长春 130023;
    2. 吉林大学理论化学研究所, 超分子结构及谱学教育部开放实验室, 长春 130023
  • 收稿日期:2000-05-12 出版日期:2001-07-24 发布日期:2001-07-24
  • 通讯作者: 李青山(1942年出生),男,教授,博士生导师,从事蛋白质与酶的研究.3现在吉林省药品检验所工作.
  • 基金资助:

    清华大学生物膜与膜生物工程国家重点实验室资助

A CD and FT-Raman Study on the Secondary Structure of Saporin-S6

WANG Yong-Ting1, HUANG Pin-Wei2, LI Zheng-Qiang2, XU Fei1, MENG Qing-Fan1, YANG Yi1, SHI Xue-Hui1, LI Qing-Shan1   

  1. 1. College of Life Science;
    2. Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China
  • Received:2000-05-12 Online:2001-07-24 Published:2001-07-24

关键词: 肥皂草素, 二级结构, 喇曼光谱, 圆二色谱法

Abstract: Saporin-S6 is purified from the seeds of Saponaria officinalis and its CDand FT-Raman spectra are determined. From the CDspectra of fresh protein aqueous and the same aqueous that was laid aside for 90 days, it is proved thatsaporin S6 has unusual stability. Throughout the FT-Raman spectra of saporin-S6, we work out that the α-helix of saporin-S6 is 26%, while β-fold is 37%, and random coil is 37%.

Key words: Saporin, Secondary structure, Raman spectra, Circular dichroism

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