高等学校化学学报

高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (7): 1120.

• 研究简报 • 上一篇    下一篇

四氨基铝酞菁作为过氧化物酶模拟酶新型红区荧光底物的研究

陈小兰1, 李东辉2, 杨黄浩1, 朱庆枝1, 郑洪1, 许金钩1   

  1. 1. 厦门大学化学系, 现代分析科学教育部重点实验室;
    2. 厦门大学抗癌研究中心, 厦门 361005
  • 收稿日期:2000-08-10 出版日期:2001-07-24 发布日期:2001-07-24
  • 通讯作者: 许金钩(1937年出生),男,教授,博士生导师,从事分子发光分析和生化分析的研究.
  • 基金资助:

    国家自然科学基金(批准号:29775021)

Studies on Tetra-substituted Amino Aluminum Phthalocyanine as a New Red-region Substrate for Mimetic Peroxidase

CHEN Xiao-Lan1, LI Dong-Hui2, YANG Huang-Hao1, ZHU Qing-Zhi1, ZHENG Hong1, XU Jin-Gou1   

  1. 1. The Key Laboratory of Analytical Sciences of MOE and Department of Chemistry;
    2. Cancer Research Center, Xiamen University, Xiamen 361005, China
  • Received:2000-08-10 Online:2001-07-24 Published:2001-07-24

PDF (PC)

被引次数

40

关键词: 氯化血红素, 四氨基铝酞菁, 荧光法, 过氧化氢

Abstract: Tetra-substituted amino aluminum phthalocyanine (TAAlPc) has been synthesized and used for the first time as a new red region fluorescentsubstrate for the determination of hydrogen peroxide catalyzed by peroxidase or mimetic peroxidase. Under optimum conditions, the calibration graph has a linear range of 0.0-3.0×10-7 mol/L H2O2 with a detection limit of 3.7×10-9 mol/L. The feasibility of TAAlPc as a new promising red region substrate in practical application has been proven in the determination of H2O2 in rainwater.The proposed method can largely minimize the interference that results from background fluorescence or scattering light and has a high analytical sensitivity.

Key words: Hemin, Tetra substituted amino aluminum phthalocyanine, Fluorimetry, Hydrogen peroxide

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